色谱

色谱 ›› 2015, Vol. 33 ›› Issue (5): 475-480.DOI: 10.3724/SP.J.1123.2015.01008

• 特别策划：新型分离材料专栏 • 上一篇    下一篇

牛血清白蛋白和溶菌酶为双模板蛋白质的表面印迹聚合物的制备及吸附性能

盖青青1, 屈锋2   

  1. 1. 北京低碳清洁能源研究所, 北京 102209;
    2. 北京理工大学生命学院, 北京 100081
  • 收稿日期:2015-01-09 发布日期:2015-04-23
  • 通讯作者: 盖青青

Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins

GAI Qingqing1, QU Feng2   

  1. 1. National Institute of Clean-and-Low-Carbon Energy, Beijing 102209, China;
    2. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
  • Received:2015-01-09 Published:2015-04-23

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138

被引次数

2

摘要：

采用反应条件温和的原子转移自由基聚合法(ATRP),以N-异丙基丙烯酰胺(NIPAAm)和丙烯酰胺(AAm)为功能单体,以N-(3-二甲氨基丙基)甲基丙烯酰胺(DMAPMA)为辅助功能单体,制备了以牛血清白蛋白(BSA)和溶菌酶(Lyz)为双模板蛋白质的表面印迹聚合物(Bi-MIP)。对印迹过程中辅助功能单体的量进行了考察,结果表明,在单一蛋白质溶液和混合蛋白质溶液中,当DMAPMA为20 μL时,制备的Bi-MIP对模板蛋白质BSA和Lyz有较好的吸附容量与选择性。通过静态吸附实验考察了Bi-MIP的吸附性能,并结合Langmuir吸附模型得到聚合物对模板蛋白质BSA和Lyz的最大吸附容量分别为10.2 mg/g和19.2 mg/g。且该印迹聚合物在实际样品中对模板蛋白质也表现出较强的吸附能力和较高的选择性。该方法将为复杂生物样品中同时对双/多种目标蛋白质的识别提供一种新的途径。

关键词: 表面分子印迹聚合物, 双模板蛋白质, 吸附性能

Abstract:

The double template surface-imprinted polymer (Bi-MIP) was synthesized by atom transfer radical polymerization (ATRP) in aqueous media, using bovine serum albumin (BSA) and lysozyme (Lyz) as the template proteins, N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) as the monomers and N-[3-(dimethylamino)propyl]-methacrylamide (DMAPMA) as the assistant of basic functional monomer. The ATRP possessed the mild reaction conditions and can be initiated at room temperature without heating and ultraviolet radiations. The preparation conditions of imprinted polymer were optimized, and the content of DMAPMA as the assistant of basic functional monomer was investigated. The results showed that Bi-MIP exhibited the good adsorption capacity and selectivity in single protein solution and in mixed protein solution when the volume of DMAPMA in the preparation process of Bi-MIP was 20 μL. The rebinding capacity of Bi-MIP was evaluated according to adsorption isotherm of the imprinted polymer. The Langmuir adsorption model was employed to describe the isotherms and revealed that Bi-MIP exhibited the maximum rebinding to BSA and Lyz at 10.2 mg/g and 19.2 mg/g, respectively. And Bi-MIP had good imprinting effect and adsorption capacity to templates BSA and Lyz from egg white and bovine serum samples. This will provide a new way for specific recognition of the double/multiple target proteins in the complex biological samples.

Key words: adsorption characteristics, double template proteins, surface molecularly imprinted polymer

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