色谱

色谱 ›› 2017, Vol. 35 ›› Issue (11): 1192-1197.DOI: 10.3724/SP.J.1123.2017.08002

• 技术与应用 • 上一篇    下一篇

半制备型液相色谱法分离纯化茶叶鲜叶中7种儿茶素类化合物

龚智宏1,2, 陈思1,2,3, 高江涛4, 李梅红1,2,3, 汪厦霞1,2, 林军1,2, 俞晓敏1,2,3   

  1. 1. 福建农林大学海峡联合研究院园艺植物生物学及代谢组学研究中心, 福建 福州 350002;
    2. 福建省海峡植物应用系统生物学重点实验室, 福建 福州 350002;
    3. 福建农林大学园艺学院, 福建 福州 350002;
    4. 福建农林大学生命科学学院, 福建 福州 350002
  • 收稿日期:2017-08-02 出版日期:2017-11-08 发布日期:2014-01-17
  • 通讯作者: 俞晓敏,Tel:(0591)83895671,E-mail:xmyu0616@sina.com
  • 基金资助:

    福建省自然科学基金（2016J01108）.

Isolation and purification of seven catechin compounds from fresh tea leaves by semi-preparative liquid chromatography

GONG Zhihong1,2, CHEN Si1,2,3, GAO Jiangtao4, LI Meihong1,2,3, WANG Xiaxia1,2, LIN Jun1,2, YU Xiaomin1,2,3   

  1. 1. Horticultural Biology and Metabolomics Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fuzhou 350002, China;
    3. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    4. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2017-08-02 Online:2017-11-08 Published:2014-01-17
  • Supported by:

    Fujian Natural Science Foundation of China (No. 2016J01108).

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摘要：

建立了从茶叶鲜叶中分离纯化7种儿茶素类化合物（没食子儿茶素（GC）、表没食子儿茶素（EGC）、儿茶素（C）、表没食子儿茶素没食子酸酯（EGCG）、表儿茶素（EC）、表没食子儿茶素3-O-（3-O-甲基）没食子酸酯（EGCG3"Me）和表儿茶素没食子酸酯（ECG））的半制备色谱法。铁观音鲜叶经甲醇超声浸提、浓缩、氯仿萃取后，向水相中加入碱式醋酸铅沉淀，得到茶多酚粗品。分别以甲醇-水、乙腈-水作为流动相，采用半制备色谱法纯化7种儿茶素类化合物，纯度均达到90%。此外，利用同样的方法分离纯化另外两种茶叶鲜叶中的7种儿茶素类化合物，得到相似的结果。该方法以溶剂提取、离子沉淀结合半制备色谱，适于简单、高效地同时分离制备多种儿茶素类化合物。

关键词: 半制备色谱, 茶叶, 儿茶素类化合物, 分离纯化, 离子沉淀

Abstract:

An effective and simple method was established to simultaneously purify seven tea catechins (gallocatechin (GC), epigallocatechin (EGC), catechin (C), epigallocatechin-3-O-gallate (EGCG), epicatechin (EC), epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG3"Me) and epicatechin-3-O-gallate (ECG)) from fresh tea leaves by semi-preparative high performance liquid chromatography (HPLC). Fresh leaves of Tieguanyin tea were successively extracted with methanol and chloroform. Then crude catechins were precipitated from the aqueous fraction of chloroform extraction by adding lead subacetate. Crude catechins were used for the isolation of the seven target catechin compounds by semi-preparative HPLC. Methanol-water and acetonitrile-water were sequentially used as mobile phases. After two rounds of semi-preparative HPLC, all target compounds were achieved with high purities (>90%). The proposed method was tested on two additional tea cultivars and showed similar results. This method demonstrated a simple and efficient strategy based on solvent extraction, ion precipitation and semi-preparative HPLC for the preparation of multiple catechins from tea leaves.

Key words: catechin compounds, ion precipitation, isolation and purification, semi-preparative high performance liquid chromatography (HPLC), tea leaves

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