色谱 ›› 2018, Vol. 36 ›› Issue (8): 758-765.DOI: 10.3724/SP.J.1123.2018.02005

• 研究论文 • 上一篇    下一篇

高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分

何嘉雯, 温家欣, 赖宇红, 曹雅静   

  1. 广东省药品检验所, 广东 广州 510180
  • 收稿日期:2018-02-04 出版日期:2018-08-08 发布日期:2014-06-28
  • 通讯作者: 何嘉雯,E-mail:hjw7hjw@163.com
  • 基金资助:

    广东省省级科技计划项目(2015A030401062).

Determination of 28 exogenous medicines and endogenous components in herbal drink using high performance liquid chromatography

HE Jiawen, WEN Jiaxin, LAI Yuhong, CAO Yajing   

  1. Guangdong Institute for Drug Control, Guangzhou 510180, China
  • Received:2018-02-04 Online:2018-08-08 Published:2014-06-28
  • Supported by:

    Science and Technology Planning Project of Guangdong Province (No.2015A030401062).

摘要:

建立了高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分的分析方法。样品经甲醇-水(70:30,v/v)超声提取后,使用Thermo Accucore C18色谱柱(100 mm×4.6 mm,2.6 μm),以甲醇、乙腈和20 mmol/L乙酸铵溶液(pH 4.2)为流动相进行梯度洗脱,流速为1.2 mL/min,柱温为35℃,采用二极管阵列检测器,检测波长为254和220 nm,外标法定量。结果表明,28种成分在1~100 mg/L范围内线性关系良好,相关系数(r)均不小于0.999。液体样品和固体样品的检出限(LODs)分别为1~10和20~200 mg/kg。28种成分在液体和固体样品中的加标回收率分别为88.8%~118.6%和92.7%~112.3%,相对标准偏差(RSDs)分别为0.1%~6.7%和0.1%~6.4%。按上述方法检测草本植物饮料456批,检出阳性样品55批次,检出率为12.1%。该法简便快速、准确可靠,适用于草本植物饮料中28种成分的测定。

关键词: 草本植物饮料, 非法添加药物, 高效液相色谱法, 核壳色谱柱

Abstract:

A high performance liquid chromatographic method for the determination of 28 exogenous medicines and endogenous components in the herbal drink was developed. The samples were extracted ultrasonically with methanol-water (70:30, v/v), and the extracts were separated in a Thermo Accucore C18 column (100 mm×4.6 mm, 2.6 μm) with methanol-acetonitrile-20 mmol/L ammonium acetate solution (pH 4.2) as the mobile phases by gradient elution. The flow rate was 1.2 mL/min and the column temperature was 35℃. The detection wavelengths were 254 nm and 220 nm. Quantification analysis was performed by the external standard method. The result showed the compounds had a good linear relationship in the range of 1-100 mg/L, and the correlation coefficients (r) were not less than 0.999. The limits of detection (LODs) of the 28 compounds were 1-10 mg/kg in the liquid sample and 20-200 mg/kg in the solid sample. The average recoveries of the 28 compounds in the liquid and solid samples were in the ranges of 88.8%-118.6% and 92.7%-112.3% with the relative standard deviations (RSDs) of 0.1%-6.7% and 0.1%-6.4%, respectively. The method was applied to analyze 456 herbal drink samples, and 55 positive samples were found. The positive rate was 12.1%. The developed method was simple and reliable, and it was suitable for the determination of 28 components in the herbal drink.

Key words: fused core particle column, herbal drink, high performance liquid chromatography (HPLC), illegally added medicine

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