色谱 ›› 2018, Vol. 36 ›› Issue (10): 947-951.DOI: 10.3724/SP.J.1123.2018.05019

• 专论与综述 • 上一篇    下一篇

复合靶指数富集的配基系统进化技术研究进展

武振宁, 薛书江, 杨咏洁   

  1. 延边大学农学院, 吉林 延吉 133002
  • 收稿日期:2018-05-23 出版日期:2018-10-08 发布日期:2014-08-22
  • 通讯作者: 杨咏洁.Tel:(0433)2435549,E-mail:yjyang@ybu.edu.cn
  • 基金资助:

    国家自然科学基金项目(31602126);吉林省教育厅基金项目(JJKH20180901KJ);延边大学青-基金项目(602017020).

Advancements in complex target systematic evolution of ligands by exponential enrichment

WU Zhenning, XUE Shujiang, YANG Yongjie   

  1. Agricultural College of Yanbian University, Yanji 133002, China
  • Received:2018-05-23 Online:2018-10-08 Published:2014-08-22
  • Supported by:

    National Natural Science Foundation of China (No. 31602126); Foundation of Educational Commission of Jilin Province (No. JJKH20180901KJ); Youth Foundation of Yanbian University (No. 602017020).

摘要:

核酸适配体是一类具有高度特异性和亲和力的单链寡核苷酸,被誉为"人工单抗",具有广阔的应用前景。它一般是通过指数富集的配基系统进化(SELEX)技术筛选获得。目前SELEX技术多局限于单一、纯化的可溶性蛋白质靶标。然而,蛋白质的纯化过程繁琐,耗时费力,而且很多靶标(如血清中的低丰度蛋白质或细胞的膜蛋白)很难纯化获得单一纯品。复合靶SELEX技术则可以避免靶标的纯化过程,能够保持靶标的天然构象,并且可以在未明确靶标的组成及结构特性的前提下,通过高通量的盲筛获得一系列特异性核酸适配体。该文主要介绍以未纯化的各种生物样本为复合靶的SELEX技术,以期为核酸适配体的筛选提供新思路。

关键词: 复合靶, 核酸适配体, 筛选, 指数富集的配基系统进化, 综述

Abstract:

Nucleic acid aptamers are single-stranded oligonucleotides that possess high affinity and specificity. They are also known as "chemical antibodies" and have a wide range of applications. Aptamers are usually generated by an in vitro process termed as systematic evolution of ligands by exponential enrichment (SELEX). Aptamers are often selected by employing purified soluble protein targets. However, the process of protein purification can be complex and laborious. Furthermore, several protein targets, such as low abundance proteins found in serums and membrane proteins found in cells, are difficult to purify. Complex target SELEX can avoid the purification step and maintain the native state of the target proteins. Even in the absence of detailed composition and characterization of a complex target, complex target SELEX can be performed with a high throughput for obtaining specific aptamers. In this study, complex target SELEX taken unpurified biological samples as targets will be described in order to provide a new idea for researchers screening aptamers.

Key words: complex targets, nucleic acid aptamers, review, screening, systematic evolution of ligands by exponential enrichment (SELEX)

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