色谱 ›› 2019, Vol. 37 ›› Issue (2): 222-226.DOI: 10.3724/SP.J.1123.2018.10040

• 研究论文 • 上一篇    下一篇

顶空-气相色谱-飞行时间质谱鉴定分析饮用水中的异味成分

蒋万枫, 张宁, 张辉珍   

  1. 青岛市食品药品检验研究院, 山东 青岛 266071
  • 收稿日期:2018-10-30 出版日期:2019-02-09 发布日期:2014-12-31
  • 通讯作者: 蒋万枫,Tel:(0532)58759169,E-mail:jwfvip@126.com
  • 基金资助:

    山东省青岛市市南区科技发展资金项目(2018-4-012-ZH).

Identification of odorous components in drinking water using headspace-gas chromatography-time-of-flight mass spectrometry

JIANG Wanfeng, ZHANG Ning, ZHANG Huizhen   

  1. Qingdao Institute for Food and Drug Control, Qingdao 266071, China
  • Received:2018-10-30 Online:2019-02-09 Published:2014-12-31
  • Supported by:

    Science and Technology Development Fund Project of Shinan District, Qingdao, Shandong Province (No. 2018-4-012-ZH).

摘要:

使用顶空-气相色谱-飞行时间质谱结合差异化分析软件鉴定分析出了饮用水中的异味物质。质谱数据通过逐级筛查,降低化合物数量,通过T检验和倍数变化统计分析查找差异显著的化合物,利用结构解析确定异味成分为乙醛。通过正交试验优化了加热时间、加热温度、样品量和加盐量等因素。检出限和定量限分别为0.0033和0.010 mg/L,线性范围为0.010~0.500 mg/L,相关系数R2=0.997。对实际样品进行了检测,分析了产生异味的原因,并对相应的生产加工工艺提出了合理化建议。该方法简便、快速、灵敏,适用于鉴定分析饮用水中的异味成分。

关键词: 差异化分析, 顶空-气相色谱-飞行时间质谱, 乙醛, 饮用水

Abstract:

The odorous compounds in drinking water were identified and analyzed by using headspace-gas chromatography-time-of-flight mass spectrometry (HS-GC-TOF-MS) combined with differential analysis software. The number of compounds were reduced by step-by-step screening of mass spectrometry data and statistical analysis of T-test and fold change wherein significant differences in compounds were observed. Further, the structural analysis method was used which revealed that the odorous component present in drinking water is acetaldehyde. The factors such as heating time, heating temperature, sample size, and salt content were optimized by orthogonal test. The limit of detection and limit of quantification were 0.0033 and 0.010 mg/L, respectively. The linear ranges were 0.010 to 0.500 mg/L, and the correlation coefficient was 0.997. Conclusively, the actual water samples were tested, the compounds producing odor were analyzed, and the reasonable suggestions for their production and subsequent processing technology were put forward. The method presented in this study is simple, rapid, sensitive, and is suitable for the identification and analysis of odorous components present in drinking water.

Key words: acetaldehyde, differential analysis, drinking water, headspace-gas chromatography-time-of-flight mass spectrometry (HS-GC-TOF MS)

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