色谱

色谱 ›› 2019, Vol. 37 ›› Issue (12): 1383-1391.DOI: 10.3724/SP.J.1123.2019.06021

• 技术与应用 • 上一篇    

高效液相色谱-紫外法快速测定塑料类食品接触材料及制品中7种对苯二甲酸酯或苯甲酸酯的特定迁移量

肖晓峰, 王建玲, 刘艇飞, 何军, 陈彤, 王吉   

  1. 台州海关综合技术服务中心, 浙江 台州 318000
  • 收稿日期:2019-06-25 出版日期:2019-12-08 发布日期:2020-12-11
  • 通讯作者: 肖晓峰.E-mail:xiao_xf@126.com.
  • 基金资助:
    浙江省分析测试科技计划项目（2018C37028）.

Rapid analysis of the specific migration of seven terephthalates and benzoates from plastic food contact material and products by high performance liquid chromatography-ultraviolet detection

XIAO Xiaofeng, WANG Jianling, LIU Tingfei, HE Jun, CHEN Tong, WANG Ji   

  1. Taizhou Customs Comprehensive Technology Service Center, Taizhou 318000, China
  • Received:2019-06-25 Online:2019-12-08 Published:2020-12-11
  • Supported by:
    Zhejiang Province Analysis and Testing Science and Technology Project (No. 2018C37028).

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摘要： 建立了高效液相色谱-紫外检测（HPLC-UV）法快速测定从塑料类食品接触材料及制品迁移至10%（v/v）乙醇、3%（m/v，即3 g/100 mL）乙酸、4%（v/v）乙酸、20%（v/v）乙醇、50%（v/v）乙醇、95%（v/v）乙醇和橄榄油7种食品模拟物中对苯二甲酸二甲酯、对苯二甲酸二辛酯、苯甲酸甲酯、苯甲酸乙酯、苯甲酸丙酯、苯甲酸丁酯和新戊二醇二苯甲酸酯的特定迁移量。考察了多种提取溶剂、QuEChERS dSPE EMR-Lipid试剂盒和Captiva EMR-Lipid试剂盒对橄榄油食品模拟物中7种对苯二甲酸酯或苯甲酸酯的提取或净化效果。以甲醇和水为流动相进行梯度洗脱，7种对苯二甲酸酯或苯甲酸酯在苯基柱上于17 min内达到基线分离。检测波长为237 nm，进样量为10 μL。7种对苯二甲酸酯或苯甲酸酯在7种食品模拟物中的定量限为0.2~8.1 mg/kg、1~80 mg/L或8~160 mg/kg，相关系数r≥0.9998。在2或8、60、80或160 mg/kg 3个加标水平的回收率为91.7%~106%，相对标准偏差为0.1%~3.1%。该方法样品前处理简便，色谱分离和线性关系好，回收率和重复性较好，已应用于实际样品的检测。

关键词: 高效液相色谱-紫外检测, 对苯二甲酸酯, 苯甲酸酯, 特定迁移量

Abstract: A method based on high-performance liquid chromatography-ultraviolet (HPLC-UV) detection was developed for the rapid analysis of the specific migration of seven terephthalates and benzoates (TPBAs) in seven food simulants (10% (v/v) ethanol, 3% (m/v, i. e. 3 g/100 mL) acetic acid, 4% (v/v) acetic acid, 20% (v/v) ethanol, 50% (v/v) ethanol, 95% (v/v) ethanol, or olive oil)). Detailed comparisons were made on the extraction or purification of the seven TPBAs in olive oil food simulants by solvents, QuEChERS dSPE EMR-Lipid technology, and Captiva EMR-Lipid technology. The seven TPBAs were separated completely within 17 min by gradient elution on a phenyl column using water and methanol as the mobile phase. The detection wavelength was set at 237 nm. The injection volume was 10 μL. The linearities of the seven TPBAs were good, with r≥0.9998 at 1-80 mg/L or 8-160 mg/kg in the seven food simulants. The average recoveries of the seven TPBAs were between 91.7% and 106%, with relative standard deviations (RSDs) between 0.1% and 3.1% (n=6). The limits of quantification were 0.2-8.1 mg/kg. This method is simple, with convenient pretreatments, good chromatographic separation and linear relationships, as well as satisfactory recoveries and RSDs. The method has been applied to detect the specific migration of the seven TPBAs in real samples.

Key words: high performance liquid chromatography-ultraviolet detection (HPLC-UV), terephthalate, benzoate, specific migration

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