毛细管电泳在手性化合物分离分析中的研究进展

doi:10.3724/SP.J.1123.2019.10019

摘要/Abstract

摘要：

手性化合物的对映异构体往往表现出不同的生理活性，因此建立手性化合物的有效分离分析方法具有重要意义。毛细管电泳（CE）是一种分离效率高、分析速度快、样品用量少、分离模式灵活多样的分离分析方法，在手性化合物的分离和检测领域应用广泛。该文主要综述了2017~2019年CE在手性分离分析方面的最新进展，并对其未来的发展趋势进行了展望。

关键词: 毛细管电泳, 手性分离, 综述

Abstract:

As the enantiomers of chiral compounds often exhibit different physiological activities, the establishment of effective separation and analytical methods for chiral compounds is of great significance. As a widely used chiral separation and detection technique, capillary electrophoresis (CE) offers the advantages of high separation efficiency, high speed, small sample consumption and flexible separation mode. In this review, research progresses in chiral separation by CE for the period 2017 to 2019 are summarized, and future developments are prospected.

Key words: capillary electrophoresis (CE), chiral separation, review

刘明霞, 李向军, 白玉, 刘虎威. 毛细管电泳在手性化合物分离分析中的研究进展[J]. 色谱, 2020, 38(3): 317-323.

LIU Mingxia, LI Xiangjun, BAI Yu, LIU Huwei. Recent advances of separation and analysis of chiral compounds by capillary electrophoresis[J]. Chinese Journal of Chromatography, 2020, 38(3): 317-323.

图/表 2

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Chiral selectors	Chiral ionic liquids	Analytes	Ref.
α-CD: α-cyclodextrin; HP-β-CD: hydroxypropyl-β-cyclodextrin.
α-CD	tetramethylammonium-L-arginine, tetraethylammonium-L-arginine, tetrabutylammonium-L-arginine, tetramethylammonium-L-proline, tetramethylammonium-L-glutamic acid, tetramethylammonium hydroxide	amlodipine, citalopram, nefopam, sulconazole, tryptophan	[22]
β-CD	tetramethylammonium-L-arginine	dansyl-tryptophan, dansyl-phenylalanine, dansyl-tyrosine, dansyl-histidine, dansyl-cysteine, dansyl-alanine	[28]
HP-β-CD	N, N, N-trimethyl-L-valinol-bis(trifluoromethanesulfon)imide, N, N-dimethyl-L-prolinol-bis(trifluoromethanesulfon)imide, N, N, N-trimethyl-L-phenylalaninol-bis(trifluoromethanesulfon)imide	laudanosine, nefopam, ketoconazole, amlodipine, sulconazole, econazole	[21]
HP-β-CD	tetramethylammonium-L-lysine, tetramethylammonium L-glutamic acid, tetrabutylammonium L-lysine, tetrabutylammonium L-glutamic acid	duloxetine, verapamil, terbutaline, econazole, sulconazole, metoprolol, nadolol	[29]
Trimethyl-β-CD, HP-β-CD	L-alanine tert butyl ester lactate	warfarin, coumachlor	[30]
γ-CD	R-N, N, N-trimethyl-2-aminobutanol-bis(trifluoromethane-sulfon)imidate	9-fluorenylmethoxycarbonyl-homocysteine	[23, 31]
Sulfated-γ-CD	tetrabutylammonium-L-aspartic acid	ivabradine	[32]
Clindamycin phosphate	tetramethylammonium-lactobionate	propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, esmolol	[24]
Dextrin	1-butyl-3-methylimidazolium(T-4)-bis[(2S)-2-(hydroxy-κO)-3-methyl-butanoato-κO]borate, 1-butyl-3-methylimidazolium(T-4)-bis[(αS)-α-(hydroxy-κO)-4-methyl-benzeneacetato-κO]borate	duloxetine, ketoconazole, sulconazole, citalopram	[25]
Maltodextrin	tertramethylammonium-D-pantothenate, tertramethylammonium-D-quinate	nefopan, ketoconazole, econazole, voriconazole	[26]
Maltodextrin	tetramethylammonium-L-arginine	nefopam, duloxetine, ketoconazole, cetirizine, citalopram	[27]
Cyclofructan-6	L-alanine tert butyl ester lactate	fexofenadine, nefopam	[30]

Chiral selectors	Chiral ionic liquids	Analytes	Ref.
α-CD: α-cyclodextrin; HP-β-CD: hydroxypropyl-β-cyclodextrin.
α-CD	tetramethylammonium-L-arginine, tetraethylammonium-L-arginine, tetrabutylammonium-L-arginine, tetramethylammonium-L-proline, tetramethylammonium-L-glutamic acid, tetramethylammonium hydroxide	amlodipine, citalopram, nefopam, sulconazole, tryptophan	[22]
β-CD	tetramethylammonium-L-arginine	dansyl-tryptophan, dansyl-phenylalanine, dansyl-tyrosine, dansyl-histidine, dansyl-cysteine, dansyl-alanine	[28]
HP-β-CD	N, N, N-trimethyl-L-valinol-bis(trifluoromethanesulfon)imide, N, N-dimethyl-L-prolinol-bis(trifluoromethanesulfon)imide, N, N, N-trimethyl-L-phenylalaninol-bis(trifluoromethanesulfon)imide	laudanosine, nefopam, ketoconazole, amlodipine, sulconazole, econazole	[21]
HP-β-CD	tetramethylammonium-L-lysine, tetramethylammonium L-glutamic acid, tetrabutylammonium L-lysine, tetrabutylammonium L-glutamic acid	duloxetine, verapamil, terbutaline, econazole, sulconazole, metoprolol, nadolol	[29]
Trimethyl-β-CD, HP-β-CD	L-alanine tert butyl ester lactate	warfarin, coumachlor	[30]
γ-CD	R-N, N, N-trimethyl-2-aminobutanol-bis(trifluoromethane-sulfon)imidate	9-fluorenylmethoxycarbonyl-homocysteine	[23, 31]
Sulfated-γ-CD	tetrabutylammonium-L-aspartic acid	ivabradine	[32]
Clindamycin phosphate	tetramethylammonium-lactobionate	propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, esmolol	[24]
Dextrin	1-butyl-3-methylimidazolium(T-4)-bis[(2S)-2-(hydroxy-κO)-3-methyl-butanoato-κO]borate, 1-butyl-3-methylimidazolium(T-4)-bis[(αS)-α-(hydroxy-κO)-4-methyl-benzeneacetato-κO]borate	duloxetine, ketoconazole, sulconazole, citalopram	[25]
Maltodextrin	tertramethylammonium-D-pantothenate, tertramethylammonium-D-quinate	nefopan, ketoconazole, econazole, voriconazole	[26]
Maltodextrin	tetramethylammonium-L-arginine	nefopam, duloxetine, ketoconazole, cetirizine, citalopram	[27]
Cyclofructan-6	L-alanine tert butyl ester lactate	fexofenadine, nefopam	[30]

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