色谱 ›› 2018, Vol. 36 ›› Issue (7): 579-587.DOI: 10.3724/SP.J.1123.2018.02021

• 专论与综述 • 上一篇    下一篇

原位衍生化技术在液相色谱和液相色谱-质谱联用分析中的应用

杜苑琪, 肖小华, 李攻科   

  1. 中山大学化学学院, 广东 广州 510275
  • 收稿日期:2018-02-20 出版日期:2018-07-08 发布日期:2014-06-03
  • 通讯作者: 肖小华,Tel:020,84111664,E-mail:xiaoxhua@mail.sysu.edu.cn;李攻科,Tel:020,84110922,E-mail:cesgkl@mail.sysu.edu.cn
  • 基金资助:

    国家自然科学基金项目(21475153,21675178,21675179);广东省自然科学基金项目(2015A030311020);广东省公益研究与能力建设专项(2015A030401036);广州市科技计划项目(201604020165).

Applications of in situ derivatization in liquid chromatography and liquid chromatography-mass spectrometry

DU Yuanqi, XIAO Xiaohua, LI Gongke   

  1. School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2018-02-20 Online:2018-07-08 Published:2014-06-03
  • Supported by:

    National Natural Science Foundation of China(No. 21475153, 21675178, 21675179);Key Project of Natural Science Foundation of Guangdong Province(No. 2015A030311020);Public Welfare Research and Capacity Building of Guangdong Province(No. 2015A030401036);Guangzhou Science and Technology Project(No.201604020165).

摘要:

衍生化是将待分析物转化为更适合的物质形式以便于分析的有效手段。原位衍生化技术作为一种常用的柱前衍生化方法,可以在样品基质中同时完成分析物的萃取和衍生化,具有高效、灵敏和选择性好的优点。原位衍生化结合其他前处理技术广泛用于胺类、醛酮类、醇类、酚类、羧酸和巯基化合物的分析中,在生物、药物、食品、环境、化妆品分析等领域有广泛的应用。该文概述了原位衍生化的反应类型和代表性衍生试剂,综述了原位衍生化技术在液相色谱和液相色谱-质谱联用分析中的应用,并展望其发展趋势。

关键词: 液相色谱, 液相色谱-质谱, 原位衍生化, 综述

Abstract:

Derivatization is an effective method to analyze substances, in which the analyte is converted into a more suitable form for analysis. As one of the most used pre-column derivatization methods, in situ derivatization can simultaneously extract and derivatize the analyte in a sample matrix, resulting in good efficiency, sensitivity, and selectivity of the analytical method. Recently, in situ derivatization combined with sample pretreatment techniques has been widely used in biological, drug, food, environmental, and cosmetic samples for the analysis of trace amines, aldehydes and ketones, alcohols, phenols, carboxylic acids, and thiols. This review summarizes the reaction types and representative derivatization reagents of in situ derivatization. The applications of in situ derivatization in liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) analysis are examined. Furthermore, the long-term prospects and potential applications of in situ derivatization are discussed.

Key words: in situ derivatization, liquid chromatography(LC), liquid chromatography-mass spectrometry(LC-MS), review

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