色谱 ›› 2019, Vol. 37 ›› Issue (3): 274-278.DOI: 10.3724/SP.J.1123.2018.11010

• 专论与综述 • 上一篇    下一篇

纳豆激酶分离纯化和酶活性测定的研究进展

法芸1,2, 张金玲3, 赵海杰2, 刘会洲1,2   

  1. 1. 中国科学院大学, 北京 100049;
    2. 中国科学院青岛生物能源与过程研究所, 山东 青岛 266101;
    3. 潍坊海关, 山东 潍坊 261041
  • 收稿日期:2018-11-08 出版日期:2019-03-08 发布日期:2015-01-21
  • 通讯作者: 刘会洲.E-mail:liuhz@qibebt.ac.cn.
  • 基金资助:

    国家自然科学基金(21405166).

Progress in separation and purification of nattokinase and its enzyme activity determination

FA Yun1,2, ZHANG Jinling3, ZHAO Haijie2, LIU Huizhou1,2   

  1. 1. University of Chinese Academy of Sciences, Beijing 100049, China;
    2. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;
    3. Weifang Customs, Weifang 261041, China
  • Received:2018-11-08 Online:2019-03-08 Published:2015-01-21
  • Supported by:

    National Natural Science Foundation of China (No. 21405166).

摘要:

该文对纳豆激酶的分离纯化和酶活性测定进行了综述。重点讨论了溶剂沉淀法、柱层析法、磁性微球吸附法、膨胀床法、反相胶束法、三相分割法等分离方法。对酶活性的不同测定方法进行了讨论和比较。提出了将核酸适配体识别技术用于纳豆激酶分离纯化和酶活性测定的可行性。

关键词: 分离纯化, 酶活性, 纳豆激酶, 综述

Abstract:

In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.

Key words: enzyme activity, nattokinase, review, separation purification

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