色谱

色谱

• 研究论文 • 上一篇    下一篇

微流控芯片电泳/电化学检测人单个肝癌细胞中的谷胱甘肽

王文雷1,2,金文睿1

  

  1. 1.School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;
    2.Shandong Province Environmental Monitoring Centre, Jinan 250013, China

  • 收稿日期:2007-06-11 修回日期:2007-08-20 出版日期:2007-11-30 发布日期:1984-12-25
  • 通讯作者: 金文睿

Determination of Glutathione in a Single Human Hepatocarcinoma Cell Using a Microfluidic Device Coupled with Electrochemical Detection

WANG Wenlei1,2, JIN Wenrui1

  

  1. 1.School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;
    2.Shandong Province Environmental Monitoring Centre, Jinan 250013, China

  • Received:2007-06-11 Revised:2007-08-20 Online:2007-11-30 Published:1984-12-25

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摘要：

采用微流控装置结合电化学检测研究了测定人单个血红细胞中谷胱甘肽(GSH)的方法。在该方法中,细胞的进样、定位、溶膜以及细胞中谷胱甘肽的转移和检测都在配有通道端安培检测器的双T形芯片中完成。单个细胞用液压导入到双T的交界面,在电泳缓冲液中毛地黄皂苷的作用下,细胞膜被穿孔。再施加直流电压,细胞被溶膜。释放出来的GSH被此直流电压电迁移至通道端并在Au/Hg电极上被检测。用校正曲线法可以定量测定单个细胞中的GSH。

关键词: 单细胞, 电化学检测, 肝癌细胞 , 谷胱甘肽, 微流控芯片

Abstract:

A method for the determination of glutathione (GSH) in a single human hepatocarcinoma cell, using a microfluidic chip coupled with electrochemical detection, was developed. In this method, the cell injection, loading, and cytolysis, as well as the transportation and detection of intracellular GSH were integrated in a microfluidic chip with a double-T injector and an end-channel amperometric detector. A single cell was loaded in the double-T injector on the microfluidic chip, using liquid pressure. The docked cell was perforated in the electrophoretic buffer containing digitonin and was lysed under a direct current electric field. The GSH from the single cell was electrokinetically transported to the separation channel and was electrochemically detected at an Au/Hg electrode. The GSH in the single cell was quantitatively determined using a calibration curve.

Key words: electrochemical detection, glutathione, hepatocarcinoma cell , single cell, microfluidic chip