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固相微萃取/加速溶剂萃取-气相色谱-质谱法分析青山绿水茶叶的挥发性成分

詹家芬1,2,陆舍铭1,孟昭宇1,向能军1,曹秋娥2,缪明明1

  

  1. 1.R. &D. Center of Hongta Tobacco Group Co., Ltd, Yuxi 653100, China; 2.School of Chemistry
    Science and Technology of Yunnan University, Kunming 650091, China
  • 收稿日期:2007-09-04 修回日期:2007-11-07 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 陆舍铭

Analysis of volatile components in Qingshanlvshui Tea using solid-phase microextraction/accelerated solvent
extraction-gas chromatography-mass spectrometry

ZHAN Jiafen1,2， LU Sheming1 ， MENG Zhaoyu1， XIANG Nengjun1，CAO Qiu’e2， MIAO Mingming1

  

  1. 1.R. &D. Center of Hongta Tobacco Group Co., Ltd, Yuxi 653100, China; 2.School of Chemistry
    Science and Technology of Yunnan University, Kunming 650091, China
  • Received:2007-09-04 Revised:2007-11-07 Online:2008-05-30 Published:1984-03-25

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摘要：

采用固相微萃取(SPME)和加速溶剂萃取(ASE)两种前处理方法从青山绿水茶叶(也叫苦丁茶)中提取挥发性成分,用气相色谱-质谱法(GC-MS)定性。实验结果表明:两种前处理方法共检测出91种成分,SPME检出49种,ASE检出56种,共同组分14种。青山绿水茶叶的挥发性成分主要成分有β-月桂烯、3,3,5-三甲基-1,5-庚二烯、L-柠檬烯、α-罗勒烯、β-罗勒烯、β-蒎烯、2-甲基安息香醛和5-羟甲基糠醛等物质。这两种提取方法各具优势,共同应用可以起到取长补短的作用。

关键词: 固相微萃取, 挥发性成分, 加速溶剂萃取, 气相色谱-质谱法, 青山绿水茶叶

Abstract:

The volatile components of Qingshanlvshui Tea were extracted using solid phase micro-extraction (SPME) and accelerated solvent extraction (ASE), and then were identified by gas chromatography-mass spectrometry (GC-MS). It showed that ninety-one compounds were identified, including forty-nine by SPME, fifty-six by ASE, and fourteen by both of them. The main constituents were β-myrcene, 3,5,5-trimethyl-1,5-heptadiene, L-limonene, α-ocimene, β-ocimene, β-pinene, 2-methylbenzaldehyde, 5-(hydroxymethyl)-2-furfural. Both SPME and ASE have their advantages. SPME is excellent at simplicity, rapidity, solvent-free, high enrichment, low detection limit, environment friendly etc. ASE has characteristics of time and solvent saving, automation, simplicity, as well as high efficiency.

Key words: accelerated solvent extraction (ASE), gas chromatography-mass spectrometry (GC-MS), Qingshanlvshui Tea , volatile components, solid phase microextraction (SPME)