色谱

• 技术与应用 • 上一篇    

丹参酚酸B生产中去除胶体类杂质的工艺方法

杨帆1, 何克江2, 刘珂1,2*   

  1. 1.Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China; 2.Shandong Target Drug Research Co. Ltd., Yantai 264006, China
  • 收稿日期:2008-09-30 修回日期:2008-11-28 出版日期:2009-05-30 发布日期:1982-09-25
  • 通讯作者: 刘珂

Removal of the colloidal impurities in the purification of salvianolic acid B

YANG Fan1, HE Kejiang2, LIU Ke1,2*   

  1. 1.Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China; 2.Shandong Target Drug Research Co. Ltd., Yantai 264006, China
  • Received:2008-09-30 Revised:2008-11-28 Online:2009-05-30 Published:1982-09-25
  • Contact: LIU Ke

摘要: 胶体类杂质的去除是丹参酚酸B(salvianolic acid B, SB)纯化工艺的难点之一。实验中基于前沿色谱与置换色谱相结合的原则,改良常规大孔吸附树脂柱的操作方法,将树脂按一定的比例装入大小两支柱。小柱与大柱串联,上样至胶体类杂质在小柱上达到吸附饱和,用50%甲醇单独洗脱大柱上的SB部分,利用树脂吸附色谱法实现了SB与胶体类杂质的分离。结果表明,经过去胶体类杂质处理后,SB的加权平均纯度由59.6%提高到64.5%,收率由69.75%提高到80.0%,并且SB的洗脱条件降低。本工艺方法适合于规模化工业生产。

关键词: 纯化 , 大孔吸附树脂, 丹酚酸B, 胶体类杂质

Abstract: The removal of the colloidal impurities in the purification of salvianolic acid B (SB) was carried out on a macroporous adsorption resin column with a modified elution method on the principle of combining frontal chromatography with displacement chromatography. Two serially connected columns were packed with macroporous adsorption resin in a certain proportion. The latter column was eluted with 50% methanol, when the colloidal impurities reached saturation adsorption in the former column. Comparative study suggested that the SB product was superior to that from the routine method with the weighted average purity risen from 59.6% to 64.5%, and the recovery from 69.75% to 80.0%. The ethanol concentration was lowered in the eluent. The method is simple and can be used for the production of SB in large scale.

Key words: colloidal impurities, macroporous adsorption resin, purification , salvianolic acid B (SB)