色谱

色谱

• 专论与综述 • 上一篇    下一篇

毛细管电色谱和加压毛细管电色谱的进展与应用

吴漪, 张晓晖, 魏娟, 薛云云, 玛尔江·巴哈提别克, 王彦, 阎超*   

  1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
  • 收稿日期:2009-07-08 修回日期:2009-07-17 出版日期:2009-09-30 发布日期:1982-03-25
  • 通讯作者: 阎超

Recent advances and applications of capillary electrochro-matography and pressurized capillary electrochromatography

WU Yi, ZHANG Xiaohui, WEI Juan, XUE Yunyun, BAHATIBIEKE Marjan, WANG Yan, YAN Chao*   

  1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2009-07-08 Revised:2009-07-17 Online:2009-09-30 Published:1982-03-25
  • Contact: YAN Chao

PDF

889

被引次数

84

摘要： 毛细管电色谱(CEC)以内含色谱固定相的毛细管为分离柱,以电渗流为驱动力,既可以分离带电物质也可以分离中性物质。它结合了毛细管电泳和高效液相色谱两者的优点,兼具高柱效、高分辨率、高选择性和高峰容量的特点,同时具有色谱和电泳的双重分离机理。然而,“纯粹”的电色谱在实际应用中有着天然的弱点,即: 在电流通过毛细管柱中的流动相时容易产生气泡(焦耳热作用),从而使电流中断和电渗流停止,毛细管柱必须被重新用流动相润湿后方能再次使用。加压毛细管电色谱(pCEC)将液相色谱中的压力流引入CEC系统中,不仅解决了气泡、干柱等问题,而且实现了定量阀进样和二元梯度洗脱。CEC和pCEC作为微分离领域的两种前沿技术,满足了当前复杂样品分析和分析仪器微型化的需求,近年来获得了广泛的关注。本文综述了这两种技术近来的发展,包括仪器、色谱固定相的发展,总结了其在生命科学、药物分析、食品安全以及环保样品分析等方面的应用进展,评述了各方法的特点,并展望了CEC和pCEC今后的发展和应用前景。

关键词: 固定相, 环境分析 , 加压毛细管电色谱, 毛细管电色谱, 生物分析, 食品分析, 药物分析

Abstract: Capillary electrochromatography (CEC), in which electroosmotic flow (EOF) created from the electrical double layer is made to act as a pump to drive the mobile phase in a capillary column packed with micro-particulates or coated with stationary phase. Both neutral and charged species can be resolved by CEC. It has been demonstrated that the efficiency of a separation obtained by electroosmotic propulsions is superior to that obtained by pressure-driven flow (as is the case in HPLC). CEC combines the best features of CE and versatile selectivity and large sample capacity of HPLC, promising high efficiency, high resolution, high selectivity and high peak capacity. However, in practice, when CEC is used without pressure, often used on a commercial CE instrument, there are problems and difficulties associated with bubbles formation and column dry-out. These difficulties can be overcome by a pressurized CEC (pCEC) system, in which a supplementary pressure is applied to the column in addition to the EOF. In such a system, a pressure can be applied to the capillary column to suppress bubbles formation. Quantitative sample introduction in pCEC can be easily achieved through a rotary-type injector. Most importantly, it is amenable for a solvent gradient mode, similar to that in HPLC, by programming the composition of mobile phase. The article brings a comprehensive survey of recent development of CEC and pCEC, including the development of instrumentation, capillary columns and stationary phase as well as CEC and pCEC applications in life science, biotechnology, pharmaceutical analysis, food safety and environmental security. Prospects for CEC and pCEC development and application are also discussed.

Key words: biochemical analysis, environmental analysis , food analysis, pharmaceutical analysis, pressurized capillary electrochromatography (pCEC), stationary phase, capillary electrochromatography (CEC)