色谱

色谱 ›› 2012, Vol. 30 ›› Issue (01): 107-110.DOI: 10.3724/SP.J.1123.2011.08055

• 技术与应用 • 上一篇    

乙醛酸和草酸的毛细管区带电泳分析

关瑾*, 王慧泽, 任丽艳, 牛秋玲   

  1. 沈阳化工大学应用化学学院, 辽宁 沈阳 110142
  • 收稿日期:2011-09-01 修回日期:2011-11-01 出版日期:2012-01-28 发布日期:2012-03-01
  • 通讯作者: 关瑾,博士,副教授,研究方向为药物分离分析新方法与新技术.
  • 基金资助:
    辽宁省自然科学基金项目(No. 20092055).

Determination of glyoxalate and oxalate by capillary zone electrophoresis

GUAN Jin*, WANG Huize, REN Liyan, NIU Qiuling   

  1. School of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China
  • Received:2011-09-01 Revised:2011-11-01 Online:2012-01-28 Published:2012-03-01

PDF

279

被引次数

12

摘要： 建立了同时测定乙醛酸和草酸的毛细管区带电泳法。考察了缓冲溶液的种类、浓度和pH以及分离电压等因素对分离结果的影响。在缓冲溶液为20 mmol/L硼砂-5.5 mmol/L邻苯二甲酸氢钾(pH 9.0)、分离电压20 kV、检测波长212 nm的优化条件下,11 min内即可实现对目标物的分离。乙醛酸和草酸分别在0.8～20 g/L和1.2～20 g/L范围内线性关系良好,相关系数分别为0.9993和0.9975;方法的检出限分别为0.2和0.4 g/L(信噪比为3);样品的加标回收率为98.3%～102.5%,相对标准偏差为0.35%～0.61%。该方法操作简便、快速、成本低廉,已应用于实际样品的分析,并获得了令人满意的结果。

关键词: 草酸, 毛细管区带电泳, 乙醛酸

Abstract: A method for the simultaneous determination of glyoxalate and oxalate by capillary zone electrophoresis (CZE) was developed. The influences of type, concentration and pH of the running buffer, and the applied voltage on separation were investigated. Glyoxalate and oxalate were separated within 11 min under the conditions of 20 mmol/L borax-5.5 mmol/L potassium hydrogen phthalate (pH 9.0), applied voltage of 20 kV, and detected wavelength of 212 nm. The calibration curves of glyoxalate and oxalate showed good linearity in the ranges of 0.8~20 g/L and 1.2~20 g/L, respectively. The correlation coefficients were 0.9993 and 0.9975, respectively. The limits of detection for glyoxalate and oxalate were 0.2 and 0.4 g/L (S/N=3), respectively. The average recoveries at three spiked levels were 98.3%~102.5% with acceptable relative standard deviations of 0.35%~0.61%. This method is simple, low cost and high performance. The method was successfully used for the determination of glyoxalate and oxalate in real samples, and the assay results were satisfactory.

Key words: glyoxalate, oxalate, capillary zone electrophoresis (CZE)