色谱

色谱 ›› 2012, Vol. 30 ›› Issue (06): 630-634.DOI: 10.3724/SP.J.1123.2012.02015

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定化妆品中7种萘二酚类物质

陈立坚, 黄金凤, 何敏恒, 林森煜, 郭新东*   

  1. 广州市质量监督检测研究院, 广东 广州 510110
  • 收稿日期:2012-02-10 修回日期:2012-03-08 出版日期:2012-06-28 发布日期:2012-06-20
  • 通讯作者: 郭新东,博士,高级工程师. Tel: (020)83187077, E-mail: gdone@21cn.com.
  • 基金资助:

    广东省地方标准计划项目(No. ［2010］ 299).

Simultaneous determination of seven naphthalenediols in cosmetics by reversed-phase high performance liquid chromatography

CHEN Lijian, HUANG Jinfeng, HE Minheng, LIN Senyu, GUO Xindong*   

  1. Guangzhou Quality Supervision and Testing Institute, Guangzhou 510110, China
  • Received:2012-02-10 Revised:2012-03-08 Online:2012-06-28 Published:2012-06-20

PDF

331

被引次数

28

摘要： 建立了反相高效液相色谱((RP-HPLC))同时测定化妆品中7种萘二酚类物质的分析方法。膏霜类、乳液类和水类样品用95%(v/v)乙醇提取,粉类样品用95%乙醇-0.1%乙酸(3:2, v/v)溶液提取,经离心、过滤后,用C18柱,以甲醇-0.1%乙酸溶液为流动相梯度洗脱分离,使用二极管阵列检测器检测,以保留时间定性,并以紫外吸收光谱图辅助定性,外标法定量。结果表明,萘二酚类物质在线性范围内线性关系良好,相关系数均不低于0.999 0,方法的定量限(以信噪比为10计)为0.5～1.2 mg/kg,添加水平为5.0～50 mg/kg时回收率为84.0%～102%,相对标准偏差(n=6)为1.3%～5.7%。该法前处理简单、回收率高、精密度好,适用于非蜡基类化妆品中萘二酚类物质的测定。

关键词: 反相高效液相色谱法, 化妆品, 萘二酚

Abstract: A novel analytical method for the simultaneous determination of seven naphthalenediols (2,6-naphthalenediol, 1,5-naphthalenediol, 1,6-naphthalenediol, 2,7-naphthalenediol, 1,7-naphthalenediol, 1,3-naphthalenediol and 2,3-naphthalenediol) in cosmetics by reversed-phase high performance liquid chromatography (RP-HPLC) with a diode array detector was established. The moisturizer, cream, emulsion and water-like cosmetic samples were extracted with 95% ethanol (v/v), and the powder-like cosmetic samples were extracted with 95% ethanol-0.1% acetic acid (3:2, v/v), then the extracts were centrifuged, filtrated, and analyzed by RP-HPLC using a C18 column, employing methanol-0.1% acetic acid aqueous solution as the mobile phases under the condition of gradient elution. The naphthalenediols were qualitatively determined by retention times, and confirmed by ultraviolet spectra. The external standard method was applied for the quantification. The results indicated that the limits of quantification (LOQs) of the seven naphthalenediols were ranged from 0.5 mg/kg to 1.2 mg/kg (S/N=10); meanwhile, the linear correlation coefficients of them were all no less than 0.9990 within the linear ranges. Their recoveries in spiked samples at the levels of 5.0-50 mg/kg were ranged from 84.0% to 102% with the relative standard deviations (RSDs) of 1.3%-5.7% (n=6). The method is simple, precise and suitable for the determination of naphthalenediols in cosmetic samples.

Key words: cosmetics, naphthalenediols, reversed-phase high performance liquid chromatography (RP-HPLC)