色谱 ›› 2015, Vol. 33 ›› Issue (9): 904-909.DOI: 10.3724/SP.J.1123.2015.08012

• 亲水作用色谱专栏 • 上一篇    下一篇

复杂样品痕量极性小分子化合物的样品前处理及分析方法研究进展

张仟春1,2, 罗夏琳1, 李攻科1, 肖小华1   

  1. 1. 中山大学化学与化学工程学院, 广东 广州 510275;
    2. 兴义民族师范学院生物与化学学院, 贵州 兴义 562400
  • 收稿日期:2015-08-10 出版日期:2015-09-08 发布日期:2015-09-09
  • 通讯作者: 李攻科, 肖小华
  • 基金资助:

    国家自然科学基金项目(21127008);高等学校博士学科点专项基金项目(20120171110001).

Progress in sample preparation and analytical methods for trace polar small molecules in complex samples

ZHANG Qianchun1,2, LUO Xialin1, LI Gongke1, XIAO Xiaohua1   

  1. 1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China;
    2. School of Biology and Chemistry, Xingyi Normal University for Nationalities, Xingyi 562400, China
  • Received:2015-08-10 Online:2015-09-08 Published:2015-09-09

摘要:

核苷、胺、氨基酸等极性小分子化合物是生物、食品、环境等领域重要的研究对象,但各种复杂基体中痕量极性小分子的分离分析需要高效的前处理介质和技术以及快速灵敏的分析方法。本文综述了硅胶材料、有机聚合物、炭材料和硼酸材料等样品前处理分离介质及反相液相色谱、亲水作用色谱等分析方法在复杂样品痕量极性小分子化合物分析中的应用,并展望了其发展趋势。

关键词: 分析方法, 复杂样品, 极性小分子, 样品前处理, 综述

Abstract:

Small polar molecules such as nucleosides, amines, amino acids are important analytes in biological, food, environmental, and other fields. It is necessary to develop efficient sample preparation and sensitive analytical methods for rapid analysis of these polar small molecules in complex matrices. Some typical materials in sample preparation, including silica, polymer, carbon, boric acid and so on, are introduced in this paper. Meanwhile, the applications and developments of analytical methods of polar small molecules, such as reversed-phase liquid chromatography, hydrophilic interaction chromatography, etc., are also reviewed.

Key words: analytical method, complex samples, polar small molecule, review, sample preparation

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