色谱 ›› 2017, Vol. 35 ›› Issue (1): 105-109.DOI: 10.3724/SP.J.1123.2016.08039

• 专论与综述 • 上一篇    下一篇

单细胞分析研究进展

石蒙1,2, 宋志花1,2, 耿旭辉1, 吴大朋1, 关亚风1   

  1. 1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023;
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2016-08-31 出版日期:2017-01-08 发布日期:2013-05-06
  • 通讯作者: 关亚风,Tel:(0411)84379590,E-mail:guanyafeng@dicp.ac.cn.
  • 基金资助:

    国家自然科学基金项目(21405157);中国科学院重点部署项目(KFZD-SW-203,SW-203-03).

Latest developments of single cell analysis

SHI Meng1,2, SONG Zhihua1,2, GENG Xuhui1, WU Dapeng1, GUAN Yafeng1   

  1. 1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-08-31 Online:2017-01-08 Published:2013-05-06
  • Supported by:

    National Natural Science Foundation of China (No. 21405157); Key Program of the Chinese Academy of Sciences (Nos. KFZD-SW-203, SW-203-03).

摘要:

单细胞分析可以揭示生命基本单元——细胞物质组成、生理行为的多样性和差异性,是当今生命分析的主流前沿技术。同时,也对分析技术的定量检测极限、分辨率和精密操控能力提出了新挑战。文章着重从单细胞分离、检测、成像等几个主要方面,综述了单细胞技术研究的最新进展,并对分离和检测技术的发展作了展望。

关键词: 成像, 单细胞, 分离, 检测, 综述

Abstract:

Single cell analysis can reveal the cellular components and physiological behavior diversity. It is on the cutting-edge of bio-analytical chemistry. Challenges on detection limit, resolution, and precise manipulation of cells are really tougher than ever before. This review includes the developments of several main techniques about separation, detection and imaging. At last, the future developments in separation and detection are discussed.

Key words: detection, imaging, review, separation, single-cell

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