毛细管电泳与化学发光检测联用方法的研究进展

doi:10.3724/SP.J.1123.2016.08043

摘要/Abstract

摘要：

毛细管电泳由于其超高的分离效率广泛应用于生物医药、环境监测、食品科学以及公共安全等领域。然而，由于毛细管电泳具有进样量较少、检测光程较短等缺点，需要与高灵敏度检测器联用实现低浓度样品的分析。化学发光检测由于其背景信号低而具有超高的灵敏度。毛细管电泳-化学发光检测联用方法将毛细管电泳的高效分离特性与化学发光检测的高灵敏性相结合，成为一种非常重要的分析方法，广泛用于化学分析、药物筛选以及环境监测等领域。该文对近年来毛细管电泳-化学发光检测联用方法的基本原理进行概述，并对其发展趋势和应用前景进行了展望。

关键词: 化学发光检测, 毛细管电泳, 综述

Abstract:

Capillary electrophoresis (CE) is considered as a powerful method in many fields, such as biopharmaceuticals, environmental, food and public security analysis, owing to its high separation efficiency. However, the injection of small sample volumes and the short optical length lead to limited sensitivity. So it is necessary to couple with high sensitivity detector to realize the low concentration sample analysis. Chemiluminescence (CL) detection is characterized by providing low background with excellent sensitivity. By coupling with CL, both the high separation efficiency of CE and the high sensitivity of CL can be achieved at the same time. So far, this method has been widely applied to chemical analysis, drug screening, and environment monitoring. In this review, we briefly introduce some developments for CE-CL systems, and then put the emphasis on the applications in the past decades. Moreover, we discuss the perspectives of CE-CL system.

Key words: capillary electrophoresis (CE), chemiluminescence (CL) detection, review

中图分类号:

O658

易方, 黄香宜, 任吉存. 毛细管电泳与化学发光检测联用方法的研究进展[J]. 色谱, 2017, 35(1): 110-120.

YI Fang, HUANG Xiangyi, REN Jicun. Recent advances in capillary electrophoresis coupled with chemiluminescence detection[J]. Chinese Journal of Chromatography, 2017, 35(1): 110-120.

参考文献

[1] Dumke J C, Nussbaum M A. Anal Chem, 2007, 79: 1262
[2] Lee T T, Yeung E S. J Chromatogr, 1992, 595: 319
[3] Evangelista R A, Liu M, Chen F A. Anal Chem, 1995, 67: 2239
[4] Zhao W F, Chen Q D, Wu R G, et al. Electrophoresis, 2011, 32: 3025
[5] Gavin P F, Ewing A G. J Am Chem Soc, 1996, 118: 8932
[6] Guo L H, Yang H H, Qiu B. Anal Chem, 2009, 81: 9578
[7] Li J J, Kelly J F, Chernushevich I, et al. Anal Chem, 2000, 72: 599
[8] Liu Y X, Huang X Y, Ren J C. Electrophoresis, 2016, 37: 2
[9] Xu X D, Zhang H Y, Shi H M, et al. Anal Biochem, 2012, 427: 10
[10] Liu B F, Ozaki M, Utsumi Y, et al. Anal Chem, 2003, 75: 36
[11] Liu Y M, Liu Y Y, Zhou M, et al. J Chromatogr A, 2014, 1340: 128
[12] Xie H Y, Wang Z R, Fu Z F. J Pharm Anal, 2014, 4(6): 412
[13] Albrecht H O. Chemical, 1928, 136: 321
[14] Hashimoto M, Tsukagoshi K, Nakajima R, et al. J Chromatogr A, 2000, 867: 271
[15] Tsukagoshi K, Obata Y, Nakajima R. J Chromatogr A, 2002, 971: 255
[16] Wang Z R, Yue H, Wang Y H, et al. Electrophoresis, 2014, 35: 1000
[17] Yu H, Xu L, You T Y. Luminescence, 2013, 28: 217
[18] Xu L, Li L B, Huang J S, et al. Talanta, 2014, 118: 1
[19] Tsukagoshi K, Okuzono N, Nakajima R. J Chromatogr A, 2002, 958: 283
[20] Lara F J, Airado-Rodríguez D, Moreno-González D, et al. Anal Chim Acta, 2016, 913: 22
[21] Zhang X F, Xuan Y L, Sun A M, et al. Luminescence, 2009, 24: 243
[22] Han S Q, Wang H L. J Chromatogr B, 2010, 878: 2901
[23] Han S Q, Wu K G. J Chin Chem Soc, 2015, 62: 73
[24] Zhu J K, Shu L, Wu M, et al. Talanta, 2012, 93: 428
[25] Mu X M, Li S T, Lu X, et al. Electrophoresis, 2014, 35: 962
[26] Tsuge K, Tanaka T, Noda K, et al. J Liq Chromatogr Related Technol, 2012, 35: 1091
[27] Zhu J K, Shu L, Zhang F, et al. Luminescence, 2012, 27: 482
[28] Guo L H, Qiu B, Chen M X, et al. Electrophoresis, 2009, 30: 1355
[29] García-Campaña A M, Lara F J, Gámiz-Gracia L, et al. TrAC-Trends Anal Chem, 2009, 28: 973
[30] Barnett N W, Hindson B J, Lewis S W. Analyst, 2000, 125: 91
[31] Xu X Q, Wang J P, Wen F Y, et al. Anal Methods, 2015, 7: 976
[32] Shi G Y, Li J, Yin Y C, et al. Luminescence, 2013, 28: 468
[33] Hassanzadeh J, Amjadi M. Luminescence, 2015, 30: 439
[34] Duan H B, Cao J T, Wang H, et al. RSC Adv, 2016, 6: 45533
[35] Su M, Wei W, Liu S Q. Anal Chim Acta, 2011, 704: 16
[36] Guo L H, Fu F F, Chen G N. Anal Bioanal Chem, 2011, 399: 3323
[37] Ren J C, Huang X Y. Anal Chem, 2001, 73: 2663
[38] Yang W P, Zhang Z J, Deng W. J Chromatogr A, 2003, 1014: 203
[39] Hara T, Okamura S, Kato J, et al. Anal Sci, 1991, 7: 261
[40] Dadoo R, Colon L A, Zare R N, et al. J High Resol Chromatogr, 1992, 15: 133
[41] Liao S Y, Chao Y C, Whang C W. J High Resol Chromatogr, 1995, 18: 667
[42] Liao S Y, Whang C W. J Chromatogr A, 1996, 736: 247
[43] Jiang H L, He Y Z, Zhao H Z, et al. Anal Chim Acta, 2004, 512: 111
[44] Lee Y T, Whang C W. J Chromatogr A, 1997, 771: 379
[45] Liu Y M, Cheng J K. J Chromatogr A, 2003, 1003: 211
[46] Lin Z A, Sun X B, Lin Y, et al. Analyst, 2013, 138: 2269
[47] Dadoo R, Seto A G, Colón L A, et al. Anal Chem, 1994, 66: 303
[48] Tsukagoshi K, Nakahama K, Nakajima R. Anal Chem, 2004, 76: 4410
[49] Wang J H, Li L M, Huang W H, et al. Anal Chem, 2010, 82: 5380
[50] Xu Q F, Ji X H, Li H G, et al. J Chromatogr A, 2010, 1217: 5628
[51] Xie H Y, Wang Z R, Kong W J, et al. Analyst, 2013, 138: 1107
[52] Zhao S L, Bai W L, Yuan H Y, et al. Anal Chim Acta, 2006, 559: 195
[53] Lara F J, García-Campaña A M, Gámiz-Gracia L, et al. Electrophoresis, 2006, 27: 2348
[54] He W W, Zhou X W, Lu J Q. J Chromatogr A, 2006, 1131: 289
[55] Lin Z A, Wu X P, Lin X C, et al. J Chromatogr A, 2007, 1170: 118
[56] Peng C F, Huo T M, Liu L Q, et al. Electrophoresis, 2007, 28: 970
[57] Liu Y M, Wang C Q, Mu H B, et al. Electrophoresis, 2007, 28: 1937
[58] Liu Y M, Jia Y X, Tian W. J Sep Sci, 2008, 31: 3765
[59] Yang Z J, Wang X L, Qin W D, et al. Anal
Chim Acta, 2008, 623: 231
[60] Bai J, Hun X, Liu Q, et al. Microchim Acta, 2008, 160: 165
[61] Han S Q. J Chromatogr B, 2009, 877: 1591
[62] Huang Y, Shi M, Zhao S L, et al. Electrophoresis, 2011, 32: 3196
[63] Su Y Y, Chen C, Hou X L, et al. Anal Methods, 2011, 3: 2893
[64] Zhao S L, Liu J W, Huang Y, et al. Chem Commun, 2012, 48: 699
[65] Ye F G, Liu J W, Huang Y, et al. J Chromatogr B, 2013, 936: 74
[66] Ye F G, Yang T Z, Huang Y, et al. Anal Methods, 2013, 5: 5657
[67] Suh Y S, Kamruzzaman M, Alam A M, et al. Luminescence, 2014, 29: 248
[68] Zhao S L, Yuan H Y, Xie C, et al. J Chromatogr A, 2006, 1107: 290
[69] Zhao S L, Bai W L, Wang B, et al. Talanta, 2007, 73: 142
[70] Zhao S L, Xie C, Lu X, et al. J Chromatogr B, 2006, 832: 52
[71] Huang Y, Zhao S L, Shi M, et al. J Pharmaceut Biomed Anal, 2011, 55: 889
[72] Huang Y, Zhao S L, Shi M, et al. Anal Biochem, 2010, 399(1): 72
[73] Zhao S L, Huang Y, Shi M, et al. Anal Biochem, 2009, 393(1): 105
[74] Zhu J K, Shu L, Wu M, et al. Journal of East China Normal University (Natural Science), 2013, 32(5): 96 朱金坤, 舒露, 吴敏, 等. 华东师范大学学报(自然科学版), 2013, 32(5): 96
[75] Wang J, Han S Q. Chromatographia, 2013, 76: 715
[76] Wang L, Liu Y, Xie H Y, et al. Electrophoresis, 2012, 33: 1589
[77] Wang J W, Zhang X J, Pi F F, et al. Electrochim Acta, 2009, 54: 2379
[78] Liu Y, Fu Z F, Wang L. Luminescence, 2011, 26: 397
[79] Li T, Wang Z R, Xie H Y, et al. J Chromatogr B, 2012, 911: 1
[80] Fu Z F, Li Z Y, Xie H Y, et al. Electrophoresis, 2010, 31: 3342
[81] Zhou S L, Wang J H, Huang W H, et al. J Chromatogr B, 2007, 850: 343
[82] Liu H Y, Han N, Zhang L Y, et al. Anal Chim Acta, 2010, 680: 48
[83] Zhang Y X, Zhang Z J, Yang F. J Chromatogr B, 2007, 857: 100
[84] Liu Y M, Mu H B, Zheng Y L, et al. J Chromatogr B, 2007, 855: 280
[85] Zhi Q, Xie C, Huang X Y, et al. Anal Chim Acta, 2007, 583: 217
[86] Zhao S L, Li X T, Liu Y M. Anal Chem, 2009, 81: 3873
[87] Zhao S L, Huang Y, Shi M, et al. Anal Chem, 2010, 82: 2036
[88] Zhao S L, Huang Y, Liu Y M. J Chromatogr A, 2009, 1216(39): 6746
[89] Ye F G, Huang Y, Xu Q, et al. Electrophoresis, 2010, 31: 1630
[90] Zhao S L, Huang Y, Ye F G, et al. J Chromatogr A, 2010, 1217: 5732
[91] Zhao S L, Niu T X, Song Y R, et al. Electrophoresis, 2009, 30: 1059
[92] Zhao S L, Lan X H, Liu Y M. Electrophoresis, 2009, 30(15): 2676
[93] Liu Y M, Mei L, Liu Y Y, et al. Electrophoresis, 2014, 35: 972
[94] Liu Y M, Mei L, Liu L J, et al. Anal Chem, 2011, 83: 1137
[95] Liu Y M, Cao J T, Liu Y Y, et al. Electrophoresis, 2015, 36: 2413
[96] Jiang J, Zhao S L, Huang Y, et al. J Chromatogr A, 2013, 1282: 161
[97] Zhao Y S, Zhao S L, Huang J M, et al. Talanta, 2011, 85: 2650
[98] Zhou Z M, Feng Z, Zhou J, et al. Sens Actuators B, 2015, 210: 158
[99] Zhang L L, Zhao Y S, Huang J M, et al. J Chromatogr B, 2014, 967: 190
[100] Zhou Z M, Feng Z, Zhou J, et al. Biosens Bioelectron, 2015, 64: 493
[101] Chen Z H, Tan Y, Xu K F, et al. Biosens Bioelectron, 2016, 75: 8
[102] Yang W P, Zhang Z J, Deng W. Anal Chim Acta, 2003, 485: 169
[103] Yang W P, Zhang Z J, Deng W. J Chromatogr A, 2003, 1014: 203
[104] Lin Z A, Xie Z H. J Sep Sci, 2008, 31: 2852
[105] Li T, Xie H Y, Fu Z F. Anal Chim Acta, 2012, 719: 82
[106] Tsukagoshi K, Matsumoto K, Ueno F, et al. J Chromatogr A, 2006, 1123: 106
[107] Ji X H, He Z K, Pang D W. Electrophoresis, 2007, 28: 3260
[108] Hu H M, Yin X F, Wang X Z, et al. J Sep Sci, 2013, 36: 713
[109] Shu L, Zhu J K, Wang Q J, et al. Luminescence, 2014, 29: 579
[110] Lin Z A, Sun X B, Hu W L, et al. Electrophoresis, 2014, 35: 993