超高效液相色谱-飞行时间-串联质谱法用于大白菜叶片中的脂质成分分析

doi:10.3724/SP.J.1123.2016.09034

色谱 ›› 2017, Vol. 35 ›› Issue (2): 169-177.DOI: 10.3724/SP.J.1123.2016.09034

超高效液相色谱-飞行时间-串联质谱法用于大白菜叶片中的脂质成分分析

郑姝宁, 张延国, 吕军, 徐东辉

中国农业科学院蔬菜花卉研究所, 农业部蔬菜产品质量安全风险评估实验室(北京), 农业部园艺作物生物学与种质创制重点实验室, 北京 100081

收稿日期:2016-09-16 出版日期:2017-02-08 发布日期:2013-06-09
通讯作者: 徐东辉,Tel:(010)82106963,E-mail:xudonghui@caas.cn
基金资助:
国家蔬菜产品质量安全风险评估项目（GJFP201601401）；食用农产品特征性营养组成识别与验证评估项目（GJFP2016015）；蔬菜产品未知危害因子识别与已知危害因子安全性评估项目（GJFP2016002）.

Lipid analysis of Chinese cabbage leaves by ultra performance liquid chromatography-time of flight-tandem mass spectrometry

ZHENG Shuning, ZHANG Yanguo, LÜ Jun, XU Donghui

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Risk Assessment Laboratory for Vegetable Product Quality and Safety of Ministry of Agriculture(Beijing), Key Laboratory of Horticultural Crop Biology and Germplasm Creation of Ministry of Agriculture, Beijing 100081, China

Received:2016-09-16 Online:2017-02-08 Published:2013-06-09
Supported by:
National Risk Assessment Project for Vegetable Product Quality and Safety (No. GJFP201601401); Identification and Verification of Characteristic Nutrient Composition of Edible Agricultural Products (No. GJFP2016015); Identification of Unknown Hazardous Factors and Safety Assessment Project for Known Hazard Factors from Vegetable Products (No. GJFP2016002).

摘要/Abstract

摘要：

建立了超高效液相色谱-飞行时间-串联质谱（UPLC-TOF-MS/MS）分析大白菜叶片中脂质的种类、结构、脂肪酸组成并检测其相对含量的方法。以Acquity UPLC^TM BEH C₈色谱柱为固定相，采用飞行时间质谱全扫描-信息关联采集-子离子扫描（TOF-MS scan-IDA-product ion scan）复合模式实现一次进样分析，同时获得脂质的一级和二级质谱信息。在大白菜叶片中共鉴定得到232种脂质，包含104种磷脂、63种糖脂和65种甘油酯。其中，磷脂、糖脂和甘油酯中的主要成分分别为磷脂酰胆碱、单半乳糖二酰甘油和甘油二酯、甘油三酯。结果表明，该方法具有灵敏度高、准确度高和高通量等优点，为植物脂质的代谢研究提供了可靠的分析技术平台，并为进一步的脂质生物学功能研究奠定了基础。

关键词: 超高效液相色谱-飞行时间-串联质谱, 大白菜, 叶片, 脂质

Abstract:

A method for the lipid analysis of Chinese cabbage leaves was developed by ultra performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS). The lipid species, structures, fatty acid compositions and relative contents in Chinese cabbage leaves were elucidated. Acquity UPLC^TM BEH C₈ column was selected as the stationary phase. A fast and efficient chromatographic separation was accomplished within 22 min. TOF-MS scan-information dependent acquisition-product ion scan was performed in ESI positive and negative ion modes to acquire high resolution MS and MS/MS spectra in one injection. Finally, 232 lipids in Chinese cabbage leaves were identified, including 104 phospholipids, 63 glycolipids and 65 glycerides. Among them, phosphatidylcholines and monogalactosyldiacylglycerols were chiefly phospholipids and glycolipids, respectively. Meanwhile, glycerides mainly contained diacylglycerols and triacylglycerols. The results demonstrated that the method for lipid analysis has high sensitivity, good accuracy and high throughput. It provides a reliable analysis platform for lipid metabolism in plants and lay a foundation for further study on biological functions of lipids.

Key words: Chinese cabbage, leaves, lipid, ultra performance liquid chromatography-time of flight-tandem mass spectrometry (UPLC-TOF-MS/MS)

中图分类号:

O658

郑姝宁, 张延国, 吕军, 徐东辉. 超高效液相色谱-飞行时间-串联质谱法用于大白菜叶片中的脂质成分分析[J]. 色谱, 2017, 35(2): 169-177.

ZHENG Shuning, ZHANG Yanguo, LÜ Jun, XU Donghui. Lipid analysis of Chinese cabbage leaves by ultra performance liquid chromatography-time of flight-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(2): 169-177.

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超高效液相色谱-飞行时间-串联质谱法用于大白菜叶片中的脂质成分分析

Lipid analysis of Chinese cabbage leaves by ultra performance liquid chromatography-time of flight-tandem mass spectrometry

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