色谱 ›› 2017, Vol. 35 ›› Issue (2): 203-210.DOI: 10.3724/SP.J.1123.2016.09019

• 研究论文 • 上一篇    下一篇

微乳液相色谱法同时测定降压中成药及保健品中添加的10种化学药物

黎亮星1,2, 赖彩云1, 郑艾妮1, 吴宏星1, 苏草茵1, 高崇凯1, 李宁1   

  1. 1. 广东药科大学, 广东 广州 510006;
    2. 中国广州分析测试中心, 广东 广州 510070
  • 收稿日期:2016-09-06 出版日期:2017-02-08 发布日期:2013-06-09
  • 通讯作者: 李宁,E-mail:13724117338@163.com
  • 基金资助:

    国家自然科学基金(81173525).

Simultaneous determination of 10 spiked chemical drugs in antihypertensive herb preparations and health foods by microemulsion liquid chromatography

LI Liangxing1,2, LAI Caiyun1, ZHENG Aini1, WU Hongxing1, SU Caoyin1, GAO Chongkai1, LI Ning1   

  1. 1. Guangdong Pharmaceutical University, Guangzhou 510006, China;
    2. China National Analytical Center(Guangzhou), Guangzhou 510070, China
  • Received:2016-09-06 Online:2017-02-08 Published:2013-06-09
  • Supported by:

    National Natural Science Foundation of China (No. 81173525).

摘要:

建立了微乳液相色谱(MELC)同时测定中成药及保健品中添加的10种降压药物的分析方法。考察了微乳流动相的组成成分(包括表面活性剂、助表面活性剂、油相的种类及质量分数)、pH值和固定相种类对分离效果的影响。同时利用星点设计-响应面优化法进一步优化微乳组成。实验得到的最佳分离条件:色谱柱为Agela Promosil C18(2)(250 mm×4.6 mm,5 μm);微乳流动相为5.6%(质量分数)十二烷基聚乙二醇醚(Brij35)-0.17%(质量分数)十二烷基硫酸钠(SDS)-5.1%(质量分数)正丁醇-0.46%(质量分数)环己烷-水(pH 3.0);流速为1.0 mL/min;检测波长为230 nm;柱温为30℃。该方法具有灵敏度高、稳定性好和环保的优点。

关键词: 保健品, 化学药物, 降压, 微乳液相色谱法, 中成药

Abstract:

A method was developed for simultaneous determination of 10 spiked chemical drugs in antihypertensive herb preparations and health foods by microemulsion liquid chromatography (MELC). The nature and mass fraction of microemulsion components (surfactant, co-surfactant, and oil phase), the pH of microemulsion, and the type of the stationary-phase were investigated. Central composite design-response surface methodology was used. The optimized conditions for the separation of the 10 spiked chemical drugs were as follows:an Agela Promosil C18(2) column (250 mm×4.6 mm, 5 μm) was used at 30℃, and the microemulsion mobile phase (pH 3.0) consisted of 5.6% (mass fraction) polyoxyethylene lauryl ether (Brij35), 0.17% (mass fraction) sodium dodecylsulfate (SDS), 5.1% (mass fraction) n-butanol, 0.46% (mass fraction) cyclohexane and water (pH 3.0). The method is efficient, sensitive and environment friendly.

Key words: antihypertensive, chemical drugs, health foods, herb preparations, microemulsion liquid chromatography (MELC)

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