色谱

色谱 ›› 2017, Vol. 35 ›› Issue (3): 252-254.DOI: 10.3724/SP.J.1123.2016.10001

• 研究快报 • 上一篇    下一篇

新型荧光分子印迹膜特异性识别和检测目标蛋白质

张鑫1,2, 姜锐1, 杨戍1, 孙立权1, 庞思平2, 罗爱芹1   

  1. 1. 北京理工大学生命学院, 北京 100081;
    2. 北京理工大学材料学院, 北京 100081
  • 收稿日期:2016-10-04 出版日期:2017-03-08 发布日期:2013-07-16
  • 通讯作者: 罗爱芹,Tel:(010)68915996,E-mail:bitluo@bit.edu.cn.

Specific recognition and detection of target proteins by new fluorescence molecularly imprinted membrane

ZHANG Xin1,2, JIANG Rui1, YANG Shu1, SUN Liquan1, PANG Siping2, LUO Aiqin1   

  1. 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
    2. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
  • Received:2016-10-04 Online:2017-03-08 Published:2013-07-16

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摘要：

制备了一种新型荧光分子印迹膜（L-半胱氨酸修饰的量子点嵌入的分子印迹膜（QDs@MIM）），并将其作为荧光人工受体用于目标蛋白质（溶菌酶）的特异性识别和检测。QDs@MIM以溶菌酶为模板分子、丙烯酰胺为功能单体、L-半胱氨酸修饰的量子点为辅助单体、N，N'-亚甲基双丙烯酰胺为交联剂，在预硅烷化的玻璃板上制备而成。在最佳条件下，QDs@MIM对溶菌酶检测的线性范围为0.1~1.0 μmol/L，吸附平衡时间为4 min，选择性因子为6.2。该方法操作简单、吸附平衡时间短、选择性高，具备作为生物传感器快速分析样品中目标蛋白质的潜力。

关键词: 蛋白质检测, 分子印迹膜, 量子点

Abstract:

A new method for producing L-cysteine-capped ZnS quantum dots (QDs) embedded molecularly imprinted membrane (QDs@MIM) was developed. The QDs@MIM was used as fluorescence artificial receptor for specific recognition and detection of target proteins. The QD@MIM was fabricated on a silylanized glass plate. Lysozyme, acrylamide, L-cysteine modified Mn2+-doped ZnS QDs and N,N'-methylenediacrylamide were used as template, functional monomer, assistant monomer and cross-linker, respectively. Under optimal conditions, the linear range for lysozyme ranged from 0.1 to 1.0 μmol/L, adsorption time was 4 min and the imprinting factor for lysozyme was 6.2. The results showed that the molecularly imprinted polymer can be used as a simple, rapid and selective biosensor to detect target proteins in biologic samples.

Key words: molecularly imprinted membranes (MIM), protein detection, quantum dots (QDs)

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