色谱

色谱 ›› 2017, Vol. 35 ›› Issue (3): 269-273.DOI: 10.3724/SP.J.1123.2016.10007

• 研究论文 • 上一篇    下一篇

鱼精蛋白-核酸适配体-金纳米技术快速检测牛奶中的卡那霉素

贾向阳1,3, 尤慧艳1, 付秀丽2   

  1. 1. 大连大学环境与化学工程学院, 辽宁 大连 116622;
    2. 烟台大学化学化工学院, 山东 烟台 264005;
    3. 中国科学院烟台海岸带研究所, 山东 烟台 264003
  • 收稿日期:2016-10-08 出版日期:2017-03-08 发布日期:2013-07-16
  • 通讯作者: 尤慧艳,E-mail:dlyhy@dicp.ac.cn;付秀丽.E-mail:fxl1984.happy@163.com.
  • 基金资助:

    国家重大科学仪器设备开发专项（2013YQ17052506）；国家自然科学基金项目（21275158）；烟台大学博士科研启动基金项目（HY15B13）.

Fast detection of kanamycin in milk by protamine-aptamer-gold nanotechnology

JIA Xiangyang1,3, YOU Huiyan1, FU Xiuli2   

  1. 1. College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China;
    2. Department of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China;
    3. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  • Received:2016-10-08 Online:2017-03-08 Published:2013-07-16
  • Supported by:

    National Key Scientific Instrument and Equipment Development Project (No. 2013YQ17052506); National Natural Science Foundation of China (No. 21275158); Doctoral Scientific Research Foundation of Yantai University (No. HY158B13).

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摘要：

基于聚阳离子鱼精蛋白与带负电的核酸适配体以及金纳米粒子之间的静电作用，发展了一种生物纳米检测技术，用于卡那霉素的检测；优化了缓冲溶液中阳离子、鱼精蛋白以及核酸适配体浓度，结果表明在20 mmol/L Na+、1 mmol/L Mg2+、2 mg/L鱼精蛋白、100 nmol/L核酸适配体条件下，卡那霉素在5~5000 nmol/L范围内与金纳米粒子的吸光度比值呈现良好的线性关系，相关系数（R2）为0.9928，方法的检出限为0.53 nmol/L。在此实验条件下，检测了牛奶中卡那霉素的含量，回收率为96%~98%，相对标准偏差为1.5%~3.2%。该方法选择性高，灵敏度好，线性范围广，显示出其应用于食品中卡那霉素检测的优势。

关键词: 核酸适配体, 金纳米粒子, 卡那霉素, 纳米生物, 牛奶, 鱼精蛋白

Abstract:

A bio-nanodetection system was developed for kanamycin based on electrostatic interaction between polycationic protamine and negatively charged aptamer or gold nanoparticles (AuNPs). The concentrations of protamine, aptamer and cations in the buffer solution were optimized. The results showed that there was a good linear correlation (R2=0.9928) between gold nanoparticles and kanamycin in the range of 5-5000 nmol/L with 20 mmol/L Na+, 1 mmol/L Mg2+, 2 mg/L protamine and 100 nmol/L aptamer. The limit of detection was 0.53 nmol/L. Under this experimental condition, the content of kanamycin in milk was detected, the recoveries were 96%-98%, and the relative standard deviations (RSDs) were 1.5%-3.2%. This method has the advantages of high selectivity, good sensitivity and wide linear range, and is useful in the detection of kanamycin in foods.

Key words: aptamer, bio-nano, gold nanoparticles, kanamycin, milk, protamine

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