色谱 ›› 2017, Vol. 35 ›› Issue (9): 987-994.DOI: 10.3724/SP.J.1123.2017.05010

• 研究论文 • 上一篇    下一篇

高效液相色谱-光电二极管阵列-高分辨质谱联用鉴定马鞭草提取物中的化学成分

孙玉明1, 王月月1, 蔡蕊1, 张华1, 王玉林2   

  1. 1. 大连理工大学化工学院, 辽宁 大连 116024;
    2. 大连医科大学基础医学院, 辽宁 大连 116044
  • 收稿日期:2017-05-19 出版日期:2017-09-08 发布日期:2017-09-15
  • 通讯作者: 王玉林,E-mail:wangyulin1971@126.com.

Identification of the chemical compositionsof Verbena officinalis L. extract by high performance liquid chromatography-photodiode array-high resolution mass spectrometry

SUN Yuming1, WANG Yueyue1, CAI Rui1, ZHANG Hua1, WANG Yulin2   

  1. 1. School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
    2. Basic Medical College, Dalian Medical University, Dalian 116044, China
  • Received:2017-05-19 Online:2017-09-08 Published:2017-09-15

摘要:

采用高效液相色谱-光电二极管阵列-高分辨质谱联用(HPLC-PDA-HRMS)技术对马鞭草中的主要化学成分进行分析和鉴定。通过综合分析所检测到化合物的色谱、一级和多级全扫描质谱数据,对马鞭草提取物中的化学成分进行分析鉴定。马鞭草提取物中检测到环烯醚萜苷类、黄酮类、三萜类、苯乙醇苷类和二萜酚类化合物等共21种化学成分,其中鼠尾草酸、鼠尾草酚、迷迭香酚、异迷迭香酚、迷迭香酸以及金合欢素-7-O-芸香糖苷6种化合物并未在其他有关马鞭草提取物鉴定的文献中出现。该方法简单快速,准确度高,为鉴定中药材真伪和品质提供了可靠的科学依据。

关键词: 高分辨质谱, 高效液相色谱, 光电二极管阵列检测, 结构鉴定, 马鞭草, 提取物, 质谱裂解

Abstract:

To investigate the main chemical compositions of Verbena officinals L. extract, a qualitative high performance liquid chromatography-photodiode array-high resolution mass spectrometry (HPLC-PDA-HRMS) method was established. The structures of the compounds detected were identified by analyzing the chromatographic profiles and the corresponding mass spectra obtained by full scan and MSn full scan. Twenty one compounds including iridoid glycosides, flavonoids, triterpenoids, phenylpropanoids and phenolic diterpenoids were identified, and six of them were not reported in other literatures about Verbena officinalis L. extract, such as carnosic acid, carnosol, rosmanol, isorosmanol, rosmarinic acid and acacetin-7-O-rutinoside. This method is simple, rapid, accurate and sensitive. It provides a reliable scientific basis for the identification of the authenticity and quality control of Chinese medicinal materials.

Key words: Verbena officinalis L., extract, high performance liquid chromatography (HPLC), high resolution mass spectrometry (HRMS), mass fragmentation, photodiode array detection, structure identification

中图分类号: