色谱 ›› 2018, Vol. 36 ›› Issue (3): 317-324.DOI: 10.3724/SP.J.1123.2017.11027

• 研究论文 • 上一篇    

Au@4-硝基苯硫酚@Ag@牛血清白蛋白内标化表面增强拉曼散射探针的制备及其在细胞拉曼成像中的应用

翟学萍1,2, 尤慧艳1   

  1. 1. 大连大学环境与化学工程学院, 辽宁 大连 116622;
    2. 中国科学院烟台海岸带研究所, 山东 烟台 264003
  • 收稿日期:2017-11-17 出版日期:2018-03-08 发布日期:2018-04-04
  • 通讯作者: 尤慧艳,E-mail:dlyhy@dicp.ac.cn
  • 基金资助:

    国家重大科学仪器设备开发专项(2013YQ17052506);国家自然科学基金项目(81573393).

Preparation of Au@4-nitrothiophenol@Ag@bovine serum albumin internal surface-enhanced Raman scattering tags and its application in cell Raman imaging

ZHAI Xueping1,2, YOU Huiyan1   

  1. 1. College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China;
    2. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  • Received:2017-11-17 Online:2018-03-08 Published:2018-04-04
  • Supported by:

    National Key Scientific Instrument and Equipment Development Project (No. 2013YQ17052506); National Natural Science Foundation of China (No. 81573393).

摘要:

制备了Au@4-硝基苯硫酚@Ag内标化表面增强拉曼散射(SERS)探针,进一步以牛血清白蛋白(BSA)置换探针表面的稳定剂十六烷基三甲基溴化铵(CTAB),发展了Au@NT@Ag@BSA内标化SERS探针。Au@NT@Ag@BSA探针保留了原探针的单分散性和高灵敏度,同时显著提高了信号稳定性和生物相容性。进一步将Au@NT@Ag@BSA探针和SMMC7721肺癌细胞共孵育,实现了细胞的探针标记和拉曼光谱成像。Au@NT@Ag@BSA内标化SERS探针在活体生物成像等方面展示了良好的应用潜力。

关键词: 表面增强拉曼散射探针, 内标化, 牛血清白蛋白, 细胞拉曼成像, 制备

Abstract:

This study explored a strategy of replacing the surface stabilizer of cetyltrimethylammonium bromide (CTAB) on the tag surface with bovine serum albumin (BSA) and developed a BSA coated Au@4-nitrothiophenol(NT)@Ag internal surface-enhanced Raman scattering (SERS) tag Au@NT@Ag@BSA. The Au@NT@Ag@BSA tag retained the monodispersity and high sensitivity of the original tag, while significantly improving the signal stability and biocompatibility. Furthermore, labeling and Raman spectroscopic imaging of SMMC7721 lung cancer cells were realized by the incubation of the cells with the Au@NT@Ag@BSA SERS tags. The results demonstrated that the proposed Au@NT@Ag@BSA internal SERS tags had good potential for in vivo imaging applications.

Key words: bovine serum albumin (BSA), cell Raman imaging, internal, preparation, surface-enhanced Raman scattering (SERS) tags

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