色谱

色谱 ›› 2018, Vol. 36 ›› Issue (5): 425-430.DOI: 10.3724/SP.J.1123.2017.11046

• 研究论文 • 上一篇    下一篇

超高效液相色谱-串联质谱法测定红薯叶中15种功效成分

周劭桓, 梁川, 唐阳, 廖艳华, 林文斯   

  1. 广西壮族自治区疾病预防控制中心, 广西 南宁 530028
  • 收稿日期:2017-11-24 出版日期:2018-05-08 发布日期:2014-03-21
  • 通讯作者: 周劭桓.Tel:(0771)2518670,E-mail:shaohuanzh@163.com
  • 基金资助:

    国家自然科学基金青-科学基金项目（201605027）.

Determination of 15 functional components in sweet potato leaves by ultra high performance liquid chromatography-tandem mass spectrometry

ZHOU Shaohuan, LIANG Chuan, TANG Yang, LIAO Yanhua, LIN Wensi   

  1. GuangXi Center for Disease Prevention and Control, Nanning 530028, China
  • Received:2017-11-24 Online:2018-05-08 Published:2014-03-21
  • Supported by:

    National Science Foundation for Young Scholars of China (No. 201605027).

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摘要：

建立了超高效液相色谱-串联质谱（UHPLC-MS/MS）快速测定红薯叶中15种功效成分的分析方法。样品用80%（v/v）甲醇水溶液提取后，采用C18色谱柱（100 mm×2.1 mm，1.8 μm）进行分离，以乙腈-0.15%（v/v）甲酸水溶液为流动相进行梯度洗脱，在电喷雾电离（ESI）源、负离子扫描模式下，通过多反应监测（MRM）模式检测各物质分子的离子峰。在各自的范围内15种功效成分线性关系良好，相关系数（r）为0.9995~0.9998；在3个加标水平下，15种化合物的回收率为82.2%~131.4%，相对标准偏差为3.6%~8.3%；方法的检出限为0.13~0.64 μg/L。该方法前处理简单，灵敏度高，适用于红薯叶中功效组分的快速分析，定量分析结果显示咖啡奎宁酸类物质是红薯叶中的主要功效成分。

关键词: 超高效液相色谱-串联质谱, 功效成分, 红薯叶

Abstract:

A method for the rapid determination of 15 functional components in sweet potato leaves by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established. The samples were extracted with 80% (v/v) methanol aqueous solution, and then separated on a C18 column (100 mm×2.1 mm, 1.8 μm) with gradient elution. The mobile phases were acetonitrile and 0.15% (v/v) formic acid aqueous solution. The multiple reaction monitoring (MRM) mode was used to detect the molecular ion peak of each component in electrospray ionization (ESI) source and negative ion scan mode. The results showed that the good linear relationships for the 15 components were obtained with correlation coefficients (r) ranging from 0.9995 to 0.9998. The recoveries of all the components ranged from 82.2% to 131.4% with relative standard deviations of 3.6%-8.3%. The method is simple, sensitive, and is suitable for the rapid analysis of the functional components in sweet potato leaves.

Key words: functional components, sweet potato leaves, ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)

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