三聚氰胺分子印迹光子晶体水凝胶膜传感器的制备及应用

doi:10.3724/SP.J.1123.2018.11017

摘要/Abstract

摘要：

该文将光子晶体与分子印迹技术结合，制备了分子印迹光子晶体水凝胶膜（MIPHs）作为光学传感器，用于样品中三聚氰胺的快速识别检测。以三聚氰胺为模板分子，通过垂直沉降自组装、填充聚合、去除模板3个步骤，制备得到了反蛋白石结构的三聚氰胺MIPHs。扫描电子显微镜的形貌表征表明，MIPHs具有高度有序的三维大孔结构。该MIPHs作为光学传感器可以将三聚氰胺的分子特异识别过程转换成光学信号，在对目标分析物分析时具有选择性高、响应快、灵敏度高的优点。此外，可以根据MIPHs的颜色变化利用图像软件分析或裸眼识别的方式实现目标分析物快速识别。实验结果表明，在最优条件下，三聚氰胺浓度为10^-11~10^-6mol/L时，MIPHs的布拉格衍射峰位移从563 nm红移到608 nm，而对三聚氰胺的结构类似物没有明显响应。

关键词: 分子印迹, 光学传感分析, 光子晶体, 三聚氰胺

Abstract:

In this paper, molecularly imprinted photonic crystal hydrogels (MIPHs) were prepared by combining photonic crystals with molecular imprinting technology. The MIPHs were used as optical sensors for the rapid reorganization and detection of melamine in water samples. In this experiment, melamine was used as a template molecule, and the MIPHs were prepared by successive self-assembly, polymerization, and template removal. Morphological characterization by scanning electron microscopy (SEM) showed that the MIPHs possessed a highly ordered three-dimensional (3D) macroporous structure containing nanocavities. As optical sensors, the MIPHs were able to transform molecular recognition events into fluorescence signals for rapid and highly selective and sensitive recognition of the target molecule. Based on color changes of the MIPHs, the target analyte could be quickly identified by analysis with image software or even by observation with the naked eye. Under optimal conditions, the Bragg diffraction peak of the MIPHs shifted from 563 to 608 nm when exposed to melamine in mass concentrations of 10^-11 to 10^-6mol/L, whereas there were no obvious peak shifts when it was exposed to structural analogues of melamine.

Key words: melamine, molecularly imprinting, optical sensing, photonic crystal

中图分类号:

O658

刘哲涵, 张鑫, 梁阿新, 孙立权, 罗爱芹. 三聚氰胺分子印迹光子晶体水凝胶膜传感器的制备及应用[J]. 色谱, 2019, 37(3): 287-292.

LIU Zhehan, ZHANG Xin, LIANG Axin, SUN Liquan, LUO Aiqin. Preparation and application of melamine molecularly imprinted photonic crystal hydrogel sensor[J]. Chinese Journal of Chromatography, 2019, 37(3): 287-292.

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