高效液相色谱法同时测定氧化型染发产品中33种染发剂

doi:10.3724/SP.J.1123.2019.01043

摘要/Abstract

摘要： 建立了高效液相色谱同时测定氧化型染发产品中33种禁限用染发剂含量的分析方法。采用Waters Atlantis^® T3 MV Kit色谱柱（250 mm×4.6 mm，5 μm），以磷酸盐缓冲液-乙腈为流动相，梯度洗脱，流速为1.0 mL/min，柱温为25℃；采用二极管阵列检测器（DAD），检测波长为235及280 nm，进样量为5 μL。结果表明，各成分在线性范围内线性关系良好，相关系数均高于0.999，各成分精密度实验相对标准偏差（RSDs）均小于2%；四氨基嘧啶硫酸盐和2，4-二氨基苯氧基乙醇盐酸盐在12 h内稳定性实验RSDs小于5%，其余31种成分在24 h内稳定性实验RSDs均小于5%；各成分在3个浓度水平下的加标回收率为77.6%~116.3%。该方法简便、快速、准确，适用于氧化型染发产品中多种染发剂的检测。

关键词: 高效液相色谱, 染发剂, 氧化型染发产品

Abstract: A method was developed for the simultaneous determination of 33 hair dyes in oxidative hair dye products by high-performance liquid chromatography (HPLC). The analytes were separated on a Waters Atlantis^® T3 MV Kit column (250 mm×4.6 mm, 5 μm) by gradient elution using phosphate buffer-acetonitrile as mobile phases. The components were detected at two different wavelengths of 235 and 280 nm using a diode array detector. The results showed that the method had good linearity with correlation coefficients exceeding 0.999. Precision was good for the 33 analytes, with the relative standard deviations (RSDs) less than 2%. Stabilities of tetraaminopyrimidine sulfate and 2,4-diaminophenoxyethanol HCl in 12 h, as well as other analytes in 24 h, were good, with RSDs less than 5%. Recoveries of the method ranged from 77.6% to 116.3% at three different concentration levels. The method proved to be simple, rapid, and accurate, suitable for the determination of various hair dyes in oxidative hair dye products

Key words: hair dyes, high performance liquid chromatography (HPLC), oxidative hair dye products

中图分类号:

O658

左雪, 邸铮, 张蓉, 邬国庆. 高效液相色谱法同时测定氧化型染发产品中33种染发剂[J]. 色谱, 2019, 37(7): 759-765.

ZUO Xue, DI Zheng, ZHANG Rong, WU Guoqing. Simultaneous determination of 33 hair dyes in oxidative hair dye products by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(7): 759-765.

参考文献

[1] Li X M, Wang Y, Bai X S. Dyestuffs and Coloration, 2016, 53(2):17 李学敏, 王瑛, 白雪松. 染料与染色, 2016, 53(2):17
[2] Wu Y. Occupation and Health, 2017, 33(16):2287 吴燕. 职业与健康, 2017, 33(16):2287
[3] Lin F, Jiang H, Zheng H. Studies of Trace Elements and Health, 2011, 28(1):60 林峰, 姜红, 郑珲. 微量元素与健康研究, 2011, 28(1):60
[4] Chang C K, Zhang X, Li X, et al. Journal of Diagnostics Concepts & Practice, 2011, 10(4):384 常春康, 张曦, 李晓, 等. 诊断学理论与实践, 2011, 10(4):384
[5] Zhong Z X, Luo Z B, Zhu B H. Chinese Journal of Inorganic Analytical Chemistry, 2012, 2(3):31 钟志雄, 罗志彬, 朱炳辉. 中国无机分析化学, 2012, 2(3):31
[6] Wang H R, Chen S B, Xu Z D. Analysis and Testing Technology and Instruments, 2018, 24(1):28 王海瑞, 陈少波, 徐振东. 分析测试技术与仪器, 2018, 24(1):28
[7] Wu G B, Hu Y, Ni H, et al. Journal of Analytical Science, 2015, 31(3):431 吴光斌, 胡阳, 倪辉, 等. 分析科学学报, 2015, 31(3):431
[8] Zheng X Y, Ouyang L Q, Dai M, et al. Journal of Instrumental Analysis, 2015, 34(1):86 郑小严, 欧阳立群, 戴明, 等. 分析测试学报, 2015, 34(1):86
[9] Pan C, Wu L, Xie Y, et al. Chinese Journal of Chromatography, 2018, 36(9):904 潘城, 吴凌, 谢勇, 等. 色谱, 2018, 36(9):904

[10] Zhu W X, Wang C J, Yang J Z, et al. Chinese Journal of Chromatography, 2012, 30(9):870 祝伟霞, 王彩娟, 杨冀州, 等. 色谱, 2012, 30(9):870
[11] Zhu R, Zhang X L, Wang J D, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2012, 48(5):526 朱然, 张旭龙, 王吉德, 等. 理化检验-化学分册, 2012, 48(5):526
[12] Dai M. China Surfactant Detergent & Cosmetics, 2017, 47(5):297 戴明. 日用化学工业, 2017, 47(5):297
[13] You F M. Chinese Journal of Chromatography, 2015, 33(1):17 游飞明. 色谱, 2015, 33(1):17

[14] Chen M, Hu L, Xu L, et al. China Surfactant Detergent & Cosmetics, 2016, 46(6):359 陈梦, 胡磊, 许立, 等. 日用化学工业, 2016, 46(6):359
[15] Lai Y, Chen H X, Lin R, et al. Chemical Journal of Chinese Universities, 2011, 32(10):2286 赖莺, 陈和秀, 林睿, 等. 高等学校化学学报, 2011, 32(10):2286
[16] Guerra E, Lamas J P, Llompart M, et al. Microchemical J, 2017, 132:308

[17] Lou C Y, Jiang L, Duan F, et al. Chinese Journal of Chromatography, 2017, 35(4):453 楼超艳, 姜磊, 段芬, 等. 色谱, 2017, 35(4):453

[18] China Food and Drug Administration. Safety and Technical Standards for Cosmetics. (2015-12-23)[2019-01-10]. http://samr.cfda.gov.cn/directory/web/WS01/images/MjAxNcTqtdoyNji6xbmruOa4vbz+LnBkZg==.pdf 国家食品药品监督管理总局. 化妆品安全技术规范. (2015-12-23)[2019-01-10]. http://samr.cfda.gov.cn/directory/web/WS01/images/MjAxNcTqtdoyNji6xbmruOa4vbz+LnBkZg==.pdf
[19] Yan W, Hu L, Zhou M H. China Surfactant Detergent & Cosmetics, 2015, 45(1):49 严巍, 胡磊, 周明昊. 日用化学工业, 2015, 45(1):49
[20] Rastogi S C. J Sep Sci, 2001, 24:173

[21] Zhu H J, Yang Y W, Zhang W Q, et al. Chinese Journal of Chromatography, 2008, 26(5):554 朱会卷, 杨艳伟, 张卫强, 等. 色谱, 2008, 26(5):554
[22] Lu X R, Du H M, Zhang Z, et al. Detergent & Cosmetics, 2015, 38 (12):31 卢晓蕊, 杜洪淼, 张哲, 等. 日用化学品科学, 2015, 38 (12):31