色谱

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固相微萃取-气相色谱/质谱测定植物叶片中的挥发性物质

王明林1,乔鲁芹2,张莉3,吴烈钧3,田洪孝3

  

  1. 1.College of Food Science, Shandong Agricultural University, Taian 271018, China; 2.Research
    Institute of
    Forest Ecology, Environment and Protection, Chinese Academy of Forest, Beijing 100091, China;
    3.Chemical Industry Research Institute of Shandong Province, Jinan 250014, China

  • 收稿日期:2005-08-19 修回日期:2006-01-17 出版日期:2006-07-30 发布日期:1986-12-25
  • 通讯作者: 王明林

Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/Mass Spectrometry with
Solid-Phase Microextraction

WANG Minglin1, QIAO Luqin2, ZHANG Li3, WU Liejun3, TIAN Hongxiao3

  

  1. 1.College of Food Science, Shandong Agricultural University, Taian 271018, China; 2.Research
    Institute of
    Forest Ecology, Environment and Protection, Chinese Academy of Forest, Beijing 100091, China;
    3.Chemical Industry Research Institute of Shandong Province, Jinan 250014, China

  • Received:2005-08-19 Revised:2006-01-17 Online:2006-07-30 Published:1986-12-25

摘要:

采用固相微萃取(SPME)方法吸附植物叶片中的挥发性物质,然后采用气相色谱/质谱法(GC/MS)分析了挥发性物
质的成分。在45 ℃水浴温度下,采用Polyacrylate(85 μm)固相微萃取头,在广口瓶中植物叶片的上方顶空吸附60
min,然后进行GC/MS分析。结果表明,植物叶片中的挥发性物质得到了很好的分离,受山楂叶螨(Tetraychus vienneis)
危害严重的植物的完好叶片中的挥发性物质均含有顺-3-己烯-1-醇乙酸酯、顺-3-己烯-1-醇丁酸酯和α-法呢烯,且含量
较大。初步确定这些物质是对山楂叶螨具有引诱作用的主要物质,从而为利用天然生物活性物质防治山楂叶螨提供了理论
依据。

关键词: 固相微萃取, 挥发性物质, 气相色谱, 植物叶片 , 质谱

Abstract:

A method for analyzing volatile constituents from plant leaves with gas
chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was
developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 ℃
in a water bath were efficiently extracted with Polyacrylate (85 μm) SPME fibers prior to perform
GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves
which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl
ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were
preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead
to identify biological species for preventing and treating Tetraychus vienneis.

Key words:
volatile constituents,
gas chromatography (GC), mass spectro-metry(MS), plant leaves , solid-phase microextraction (SPME)