Determination of four hydroxyl polycyclic aromatic hydrocarbons in urine by solid-phase extraction with monolithic column coupled with high performance liquid chromatography

doi:10.3724/SP.J.1123.2017.11018

Abstract

Abstract:

A method based on monolithic column solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) was developed for the simultaneous determination of four hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) in urine.A poly (butyl methacrylate-co-ethylene dimethacrylate) monolithic column was prepared in a syringe and was used as the sorbent.The parameters influencing SPE, such as loading volume, washing solvent, eluent, and elution volume, were investigated in detail.Under the optimized conditions, the linearities were obtained in range of 1.2-200.0 ng/mL, and the LODs and LOQs of the analytes were 0.06-0.09 ng/mL and 0.20-0.30 ng/mL, respectively.The intra-day and inter-day relative standard deviations were 1.4%-5.3% and 2.6%-7.3%, respectively.The recoveries evaluated using the spiked (3 ng/mL) urine samples of coke oven workers ranged from 78.2% to 117.0%.The feasibility of the developed method was further demonstrated for the analysis of the real samples.The results indicated that the reusable monolithic column enabled effective purification and enrichment of the four OH-PAHs in urine, and can be applied to the analysis of OH-PAHs in urine due to its simplicity and accuracy.

Key words: high performance liquid chromatography (HPLC), hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs), polymer monolithic column, solid-phase extraction (SPE), urine

CLC Number:

O658

HU Guiyu, WANG Manman, NIAN Qixun, LI Na, DONG Xinxin, HAO Yulan, WANG Qian, WANG Xuesheng. Determination of four hydroxyl polycyclic aromatic hydrocarbons in urine by solid-phase extraction with monolithic column coupled with high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2018, 36(4): 370-375.

References

[1] Pang Y H, Ma Y, Cui Y, et al. Journal of Environment and Health, 2012, 29(6):567 庞月红, 马芸, 崔燕, 等. 环境与健康杂志, 2012, 29(6):567
[2] Watabe Y, Nazuka N, Tezuka M, et al. Biol Pharm Bull, 2010, 33(3):389
[3] Zhao B, Li Y Q, Zhang S K, et al. Chinese Journal of Chromatography, 2014, 32(9):960 赵波, 黎玉清, 张素坤, 等. 色谱, 2014, 32(9):960
[4] Xue S W, Tang M Q, Xu L, et al. J Chromatogr A, 2015, 1411:9
[5] Yang S B, Li J, Zhang C H, et al. Chinese Journal of Ecology, 2015, 34(6):1675 杨少博, 李江, 张春辉, 等. 生态学杂志, 2015, 34(6):1675
[6] Shi L K, Liu Y L, Wang Y H, et al. Modern Food Science and Technology, 2015, 31(4):311 石龙凯, 刘玉兰, 王莹辉, 等. 现代食品科技, 2015, 31(4):311
[7] Ma J, Teng Y, Lu Y G, et al. Journal of Instrumental Analysis, 2016, 35(8):968 马军, 滕应, 陆引罡, 等. 分析测试学报, 2016, 35(8):968
[8] Ding C M, Jin Y L, Lin S B. Chinese Journal of Analytical Chemistry, 2012, 40(3):397 丁昌明, 金银龙, 林少彬. 分析化学, 2012, 40(3):397
[9] Wang Y, Dong Y L, Fan R F, et al. Journal of Environment and Health, 2006, 23(1):76 王宇, 董玉莲, 范瑞芳, 等. 环境与健康杂志, 2006, 23(1):76
[10] Huang W, Ding J, Feng Y Q. Chinese Journal of Analytical Chemistry, 2012, 40(6):830 黄维, 丁俊, 冯钰琦. 分析化学, 2012, 40(6):830
[11] You F, Zhu L, He L, et al. Chinese Journal of Analytical Chemistry, 2014, 42(12):1723 游钒, 朱岚, 何玲, 等. 分析化学, 2014, 42(12):1723
[12] Kai L, Gao Q, Hu J. J Chromatogr A, 2015, 1379:51
[13] Zhu Y S, Yang H G, An C X, et al. Chinese Journal of Analysis Laboratory, 2012, 31(10):67 朱裕穗, 杨红国, 安彩秀, 等. 分析试验室, 2012, 31(10):67
[14] Fan R, Ramage R, Wang D, et al. Talanta, 2012, 93(11):383
[15] Song W W, Luo X L, Li G K, et al. Chinese Journal of Chromatography, 2017, 35(1):20 宋雯雯, 罗夏琳, 李攻科, 等. 色谱, 2017, 35(1):20
[16] Wang J B, Wu F L, Zhao Q. Chinese Journal of Chromatography, 2015, 33(8):849 王家斌, 吴芳玲, 赵琦. 色谱, 2015, 33(8):849
[17] Li G Q, Ma G H. Chinese Journal of Chromatography, 2011, 29(7):606 李广庆, 马国辉. 色谱, 2011, 29(7):606
[18] Li X, Wang M M, Zheng G Y, et al. J Agric Food Chem, 2015, 63:4096