[an error occurred while processing this directive]
Chinese Journal of Chromatography
   Home   |   About Journal   |   Editorial Board   |   Impact Factor   |   Indexed In   |   Aims and Scope   |   Download   |   Subscriptions   |   FAQ   |   Contact Us   |   Chinese
Chinese Journal of Chromatography  2007, Vol. 25 Issue (1): 75-79    DOI:
Articles Current Issue | Next Issue | Archive | Adv Search  |   

Analysis of Monosaccharides and Uronic Acids in Polysaccharides
by Pre-column Derivatization with p-Aminobenzoic Acid
and High Performance Liquid Chromatography

HAO Guitang1, CHEN Shangwei2, ZHU Song2, YIN Hongping3, DAI Jun2, CAO Yuhua1

1.School of Chemistry and Material Engineering of Southern Yangtze University, Wuxi 214036, China;
2.Testing & Analysis Center of Southern Yangtze University, Wuxi 214036, China; 3.School of
Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
 Download: [PDF 1619KB]   [Full Text(HTML)] ()   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 

An ion-pair reversed-phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of carbohydrate and uronic acids was developed. p-Aminobenzoic acid (p-AMBA) was used for pre-column derivatization of the analytes, enabling fluorescence (λex=313 nm, λem=358 nm) or ultraviolet (UV at 303 nm) detection. Reaction conditions such as reaction temperature and reaction time were optimized. Atlantis dC18 column with hydrophilic end capping was selected for the separation of derivatives. Effects of mobile phase compositions such as ion pairs and their concentrations and pH on the retention behaviors and separation results of 9 monosaccharides and 2 uronic acids were investigated. Derivatives of fructose, galactose, glucose, mannose, xylose, arabinose, ribose, galacturonic acid, fucose, glucuronic acid and rhamnose were separated within 42 min, applying tetrabutyl ammonium hydrogen bisulfate (TBAHSO4) as the ion pair reagent. The detection limits were between 3.38×108 mol/L and 176×108 mol/L for fluorescence detection and between 2.55×107 mol/L and 13.4×107mol/L for UV detection. Good linearities were obtained with correlation coefficients (r2) above 0.99. The relative standard deviations (RSDs) of the peak area of the derivatives in 1251 h after derivatization were from 2.5% to 3.9%. This method has been applied for the determination of mono-/disaccharides and uronic acids in spirulina polysaccharide after dissolved in trifluoroacetic acid solution (2 mol/L). The results showed this method is suitable for the analysis of monosaccharide compositions in polysaccharides.

Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors

HAO Guitang

CHEN Shangwei
ZHU Song
YIN Hongping
DAI Jun
CAO Yuhua

Key wordspre-column derivatization   high performance liquid chromatography (HPLC)   p-aminobenzoic acid   monosaccharide   uronic acid   polysaccharides      
Received: 2006-05-16; Published: 2007-01-30
Corresponding Authors: DAI Jun   
Cite this article:   

HAO Guitang, CHEN Shangwei, ZHU Song et al.

Analysis of Monosaccharides and Uronic Acids in Polysaccharides
by Pre-column Derivatization with p-Aminobenzoic Acid
and High Performance Liquid Chromatography

[J]. Chinese Journal of Chromatography, 2007, 25(1): 75-79.
 
No references of article
[1] WANG Jue, JIN Yibao, WANG Tiejie, LI Xiaofan. Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection[J]. Chinese Journal of Chromatography, 2015, 33(8): 809-815.
[2] ZHANG Liyuan, YAO Di, LI Na, ZHANG Hanqi, YU Aimin. Determination of five triazine herbicides in infant milk powder by high performance liquid chromatography coupled with ionic liquid-based homogeneous liquid-liquid microextraction[J]. Chinese Journal of Chromatography, 2015, 33(7): 753-758.
[3] HU Beizhen, DONG Wenhong, XIA Biqi, SONG Weihua, HAN Chao. Simultaneous determination of monosaccharide and oligosaccharide in rice wine by high performance anion exchange chromatography with integrated pulsed amperometric detection and the establishment of their fingerprints[J]. Chinese Journal of Chromatography, 2015, 33(6): 662-666.
[4] XU Lili, ZHONG Minghua, CHEN Xiaojing. Preparation and chromatographic performance of a eugenol-bonded silica gel stationary phase for high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(5): 461-467.
[5] YE Xiaolan, SONG Fenyun, FAN Guorong, WU Fuhai. Simultaneous determination of 11 constituents in Citrus reticulate ‘Chachi’ by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(4): 423-427.
[6] HUANG Xiaoya, ZHONG Yuan, HUANG Zhongping, JIN Chen, WANG Lili, PAN Zaifa. Simultaneous determination of five active components of compound α -ketoacid tablet in human urine by ion-pair reversed-phase high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(2): 169-173.
[7] GAN Wei, LIU Xuemin, SUN Jing. Regression evaluation index intelligent filter method for quick optimization of chromatographic separation conditions[J]. Chinese Journal of Chromatography, 2015, 33(2): 140-145.
[8] ZHOU Rong, CAO Zhaoyun, MOU Renxiang, LI Zhengxiang, CHEN Mingxue. Determination of seven biothiols in rice by high performance liquid chromatography-fluorescence detection with pre-column derivatization[J]. Chinese Journal of Chromatography, 2015, 33(1): 35-39.
[9] LUO An, WAN Qiang, FAN Huajun, CHEN Zhi, WU Xuehao, HUANG Xiaowen, ZANG Linquan. Chiral separation of five arylpropionic acid drugs and determination of their enantiomers in pharmaceutical preparations by reversed-phase high performance liquid chromatography with cellulose-tris- (4-methylbenzoate) stationary phase[J]. Chinese Journal of Chromatography, 2014, 32(9): 1013-1018.
[10] SHAN Guoqiang, YU Mengqi, YU Shengsong, ZHU Lingyan. Analysis of perfluorooctanoic acid by high performance liquid chromatography with 3,4-dichloroaniline derivatization[J]. Chinese Journal of Chromatography, 2014, 32(9): 942-947.
[11] ZHAO Yunfeng, SONG Jiaxin, SUN Jiayi, YUAN Xiaowei, GUO Xingjie. Chiral separation of six tetralone derivative enantiomers using immobilized cellulose chiral stationary phase[J]. Chinese Journal of Chromatography, 2014, 32(8): 832-836.
[12] ZHAO Xinying, QU Feng, QIN Hao, LUO Aiqin. Preliminary study of two steps aqueous two-phase extraction combined with high performance liquid chromatography for saliva protein selective separation[J]. Chinese Journal of Chromatography, 2014, 32(6): 600-603.
[13] CHEN Qian, ZHOU Yuyan, CHENG Yuxiao, LIN Miao. Simultaneous determination of six aldehydes in water-based coatings by high performance liquid chromatography with pre-column derivatization[J]. Chinese Journal of Chromatography, 2014, 32(3): 230-234.
[14] WANG Xing, CHI Yumei, KANG An. Qualitative and quantitative analysis of the amino acids in Rhizoma Arisaematis by ultra high performance liquid chromatography-tandem mass spectrometry and high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2014, 32(12): 1326-1332.
[15] LIANG Tu, FU Qing, XIN Huaxia, LI Fangbing, JIN Yu, LIANG Xinmiao. Structural characterization of Astragalus polysaccharides using partial acid hydrolysis-hydrophilic interaction liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(12): 1306-1312.
?

Copyright © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS   辽ICP备10003855号
457 Zhongshan Road,116023, Dalian,P.R.China
Tel: +86-411-84379021, Fax: +86-411-84379600    E-mail: sepu@dicp.ac.cn

Supported by:Beijing Magtech

美女图片

美女

美女图片