Chinese Journal of Chromatography

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Determination of avermectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry

YANG Fang1*, YANG Shoushen2, LIN Yonghui1, ZHENG Danping2, LU Shengyu1, HUANG Zhiqiang3, ZHANG Ying3   

  1. 1.Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China; 2.Fujian Agriculture and Forestry University, Fuzhou 350003, China; 3.Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410007, China
  • Received:2008-09-03 Revised:2008-11-11 Online:2009-03-30 Published:1982-12-25
  • Contact: YANG Fang1

Abstract: A simple and rapid analytical method for the simultaneous determination of abamectin, emamectin, eprinomectin, ivermectin, doramectin and moxidectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC/ESI-MS/MS) has been developed. The avermectins were extracted from the tea with acetonitrile after the tea was infiltrated in saturated aqueous NaCl solution, then cleaned up with a C18 solid phase extraction cartridge. The linear ranges were 2.0-50 μg/L and the correlation coefficients were all above 0.9920. Several UPLC-MS/MS conditions that included the mobile phase, monitor ions and the selection of calibration of the measurement were studied . The average recoveries and the relative standard deviations ranged from 61.7% to 85.4% and from 9.37% to 17.19%, respectively, in spiked samples at the concentrations of 5, 10, 20 μg/kg for moxidectin and 2, 5, 10 μg/kg for other analytes. This method is suitable for the determination of avermectin residues in tea.

Key words: avermectin residues, tea , ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)