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Chinese Journal of Chromatography
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Chinese Journal of Chromatography  2011, Vol. 29 Issue (01): 83-86    DOI: 10.3724/SP.J.1123.2011.00083
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Determination of nucleosides in Rhizoma Pinelliae by high performance liquid chromatography
LU Dan, LUO Fen, CHI Yumei*, WU Hao
Nanjing University of Chinese Medicine, Nanjing 210046, China
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Abstract A high performance liquid chromatographic (HPLC) method was established to determine nucleosides in Rhizoma Pinelliae, which is a dried stem tuber of Pinellia pedatisecta Schott in Pinellia plant belonging to Araceae family and has multiple efficiencies about downbear counterflow and check vomiting, eliminating dampness and phlegm, etc. The separation of adenine, hypoxanthine, xanthine, uridine, thymine, adenosine and guanosine was achieved on a Lichrospher C18 column (150 mm×4.6 mm, 5 μm) with the detection at 254 nm and gradient elution by acetonitrile-water containing 0.1% formic acid as the mobile phase. The linear ranges were from 1.6 mg/L to 50 mg/L for adenine, hypoxanthine, xanthine, uridine and guanosine, while from 1.2 mg/L to 40 mg/L for thymine and adenosine with correlation coefficients above 0.9995. The average recoveries were between 98.9% and 101.2% with the relative standard deviations below 3%. The results of methodological study demonstrated that the method met the requirements of the determination. The nucleosides in Rhizoma Pinelliae from different districts were determined. The method is convenient and accurate with good reproducibility and can be used to evaluate the quality of Rhizoma Pinelliae.
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LU Dan
LUO Fen
CHI Yu-Mei
Key wordshigh performance liquid chromatography (HPLC)   nucleosides   Rhizoma Pinelliae; traditional Chinese medicinal materials     
Received: 2010-09-06; Published: 2011-01-21
Cite this article:   
LU Dan,LUO Fen,CHI Yu-Mei. Determination of nucleosides in Rhizoma Pinelliae by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2011, 29(01): 83-86.
 
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