Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2013, Vol. 31 ›› Issue (6): 582-586.DOI: 10.3724/SP.J.1123.2012.12023

• Articles • Previous Articles     Next Articles

Determination of K-ras gene mutation in colorectal cancer tissue by capillary electrophoresis

SHI Dongqin1,2, WANG Rong1,2, XIE Hua1, TIAN Wei2, JIA Zhengping1, GUO Jiankui1   

  1. 1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou 730050, China;
    2. Forest and Biotechnology College, Zhejiang Agriculture and Forest University, Lin’an 311300, China
  • Received:2012-12-12 Revised:2013-01-14 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2012.12023

PDF

155

Abstract:

The codons 12/13 of K-ras genomic DNA from 76 colorectal cancer tissues and normal tissues were amplified by PCR. The amplified 152 samples were purified, denatured, and then detected using capillary electrophoresis (CE)-laser induced fluorescence (LIF) with single-strand conformation polymorphism (SSCP). The abnormal samples were further confirmed by direct-sequencing. The 30 patients of the 76 colorectal cancer patients were found gene mutation, of which the results of base G→A point mutation were confirmed by the gene sequence. To detect K-ras gene mutation that has important role in clinical forecast, diagnosis, therapy and prognosis, the CE-LIF with SSCP was applied for detecting K-ras gene. It becomes a promising tool to analyse K-ras gene mutation in the clinical diagnosis and therapy of colorectal cancer tissue.

Key words: capillary electrophoresis (CE), colorectal cancer tissue, gene mutation, K-ras gene, single-strand conformation polymorphism

CLC Number: