Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C30 column

doi:10.3724/SP.J.1123.2013.08019

Abstract

Abstract:

A rapid and easy assay method for four triterpenoic acid isomers in Eriobotryae folium was established. The sample was extracted with methanol, then separated on a C₃₀ column (250 mm×4.6 mm, 5 μm) at 20 ℃ using acetonitrile-water (95:5, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The target compounds were simultaneously detected at 210 nm, and the injection volume was 10 μ L. The validation was carried out and the resolution (R≥2.2), the precision (RSD≤1.1%), the linearity (r≥0.9992), the repeatability (RSD≤4.4%), and the recovery (range from 95.4% to 101.7%, RSD≤4.8%) were acceptable. The method is reliable for the quantification of the four triterpenoic acid isomers by HPLC on C₃₀ column.

Key words: Eriobotryae folium, C₃₀ column, corosolic acid, high performance liquid chromatography (HPLC), isomers, maslinic acid, oleanolic acid, ursolic acid

CLC Number:

O658

ZHANG Yong, XUE Kunpeng, ZHOU An, YU Huimin, LI Yin, YAO Lixin, ZHAO Yuexing, XIE Xiaomei. Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column[J]. Chinese Journal of Chromatography, 2014, 32(1): 47-51.

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Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column

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