Determination of perfluoroalkyl acids in lamb liver by high performance liquid chromatography-tandem mass spectrometry combined with dispersive solid phase extraction

doi:10.3724/SP.J.1123.2014.12034

Abstract

Abstract:

A method was developed for the determination of 19 perfluoroalkyl acids (PFAs) in lamb liver by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with dispersive solid phase extraction. The sample was extracted with acidified acetonitrile, and then cleaned-up by a mixture of N-propylethylenediamine (PSA), C18 and graphitized carbon black (GCB) sorbents. The 19 PFAs were analyzed by HPLC-MS/MS with a C18 chromatographic column, adopting the multiple reaction monitoring (MRM) mode with negative electrospray ionization. The effects of the dosages of hydrochloric acid and the sorbents on the recoveries of the 19 PFAs were studied. For accurate quantitative analysis, the isotope internal standard method was used. The calibration curves were linear with the correlation coefficients over 0.998 in the range of 0.05-20 μg/kg for the 19 PFAs. The limits of detection were 0.004-0.111 μg/kg. The limits of quantification were 0.012-0.370 μg/kg. The mean recoveries of the 19 PFAs at spiked levels of 0.5, 1.0, 2.0 μg/kg were in the range from 80% to 128% with the relative standard deviations of 0.31%-11.1%. The developed method is rapid, simple, accurate. It is suitable for the determination of the 19 PFAs in large quantities of lamb liver samples.

Key words: dispersive solid phase extraction, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), lamb liver, perfluoroalkyl acids

CLC Number:

O658

ZHU Pingping, YUE Zhenfeng, ZHENG Zongkun, ZHANG Yi, LI Wenyin, ZHAO Fengjuan, XIAO Chengui, BAI Runye, LIN Wei. Determination of perfluoroalkyl acids in lamb liver by high performance liquid chromatography-tandem mass spectrometry combined with dispersive solid phase extraction[J]. Chinese Journal of Chromatography, 2015, 33(5): 494-500.

References

[1] Kissa E. Fluorinated Surfactants and Repellants. New York: Marcel Dekker Inc, 2001
[2] Sedlak M D, Greig D J. J Environ Monit, 2012, 14(1): 146
[3] Loi E I H, Yeung L W Y, Taniyasu S, et al. Environ Sci Technol, 2011, 45(13): 5506
[4] Plumlee M H, Larabee J, Reinhard M. Chemosphere, 2008, 72(10): 1541
[5] So M K, Taniyasu S, Lam P K S, et al. Arch Environ Contam Toxicol, 2006, 50: 240
[6] Shen B Z, Cao W J, Zhong J. Chemistry & Bioengineering (沈葆真, 曹文军, 钟键. 化学与生物工程), 2014, 31(1): 6
[7] Kato K, Wong L Y, Jia L T, et al. Environ Sci Technol, 2011, 45(19): 8037
[8] Zhang T, Sun H W, Wu Q, et al. Environ Sci Technol, 2010, 44(9): 3572
[9] Zhang T, Sun H W, Lin Y, et al. J Agric Food Chem, 2011, 59(20): 11168
[10] Hu G C, Zheng H, Zhang L J, et al. China Environmental Science (胡国成, 郑海, 张丽娟, 等. 中国环境科学), 2013, 33(S1): 37
[11] Halldorsson T I, Fei C, Olsen J, et al. Environ Sci Technol, 2008, 42(23): 8971
[12] Law R J, Bersuder P, Mead L K, et al. Marine Poll Bull, 2008, 56: 792
[13] Luque N, Leeuwen S V, Rubio S, et al. J Chromatogr A, 2010, 1217: 3774
[14] Zhang T, Sun H W, Wu Q, et al. Environ Sci Technol, 2010, 44(9): 3572
[15] Wang J M, Wang L, Feng Y J, et al. Food Science (王杰明, 王丽, 冯玉静, 等. 食品科学), 2010, 31(4): 127
[16] Li W Y, Yue Z F, Zheng Z K, et al. Journal of Food Safety and Quality (黎文茵, 岳振峰, 郑宗坤, 等. 食品安全质量检测学报), 2014, 5(1): 210
[17] Guo M M, Wu H Y, Li Z X, et al. Chinese Journal of Analytical Chemistry (郭萌萌, 吴海燕, 李兆新, 等. 分析化学), 2013, 41(9): 1322
[18] Pan Y Y, Shi Y L, Cai Y Q. Chinese Journal of Analytical Chemistry (潘媛媛, 史亚利, 蔡亚岐. 分析化学), 2008, 36(10): 1321
[19] Urairat K, Kunaporn S, Natchanun L. Anal Chim Acta, 2008, 626: 10
[20] Wang L Z, Zhou Y, Huang X Y, et al. Chinese Journal of Chromatography (王连珠, 周昱, 黄小燕, 等. 色谱), 2013, 31(12): 1167
[21] Liu Y, Tang Y F, Song J F, et al. Chinese Journal of Chromatography (刘永, 唐英斐, 宋金凤, 等. 色谱), 2014, 32(2): 139
[22] Sun Y M, Liu Y J, Zhao J, et al. Meat Research (孙亚米, 刘勇军, 赵建, 等. 肉类研究), 2014, 28(4): 14
[23] Li J, Zhang H, Chai Z F, et al. Journal of Instrumental Analysis (李静, 张鸿, 柴之芳, 等. 分析测试学报), 2014, 33(10): 1109
[24] Lehotay S J, Son K A, Kwon H, et al. J Chromatogr A, 2010, 1217: 2548
[25] Liu L Z, Guo X D, Fang J, et al. Journal of Instrumental Analysis (刘莉治, 郭新东, 方军, 等. 分析测试学报), 2013, 32(7): 862