[an error occurred while processing this directive]
Chinese Journal of Chromatography
   Home   |   About Journal   |   Editorial Board   |   Impact Factor   |   Indexed In   |   Aims and Scope   |   Download   |   Subscriptions   |   FAQ   |   Contact Us   |   Chinese
Chinese Journal of Chromatography  2015, Vol. 33 Issue (7): 699-703    DOI: 10.3724/SP.J.1123.2015.03017
Current Issue | Next Issue | Archive | Adv Search  |   
Determination of N6-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry
TANG Chunlan1,2, WANG Li1, CHENG Mengchun1, LIU Xinxin1, XIAO Hongbin1
1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
 Download: [PDF 785KB]   [Full Text(HTML)] ()   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 

An ultra performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS) method was developed and validated for the determination of N6-(4-hydroxybenzyl) adenine riboside and its pharmacokinetics in rat plasma. The chromatographic conditions were optimized. The separation was performed on an Agilent ZORBAX SB-C18 column(150 mm×3 mm, 1.8 μm) with a gradient elution of 0.2%(v/v) formic acid aqueous solution and acetonitrile as the mobile phases at a flow rate of 0.35 mL/min. The detection was accomplished in positive mode with electrospray ionization (ESI) by UPLC-QTOF-MS, and 6-benzylamino purine was used as the internal standard (IS). The results showed that the linear range of calibration curve was 0.625-160 ng/mL for N6-(4-hydroxybenzyl) adenine riboside in rat plasma with the correlation coefficient more than 0.99. The recoveries were 88.41%-108.26%. The limit of detection was 0.1 ng/mL. The intra-day and inter-day precisions (RSDs) were less than 6%, and intra-day and inter-day accuracies (REs, RE=(measured concentration-spiked concentration)/spiked concentration×100%) were less than ±15%. The method is rapid, sensitive and accurate for the quantitation of N6-(4-hydroxybenzyl) adenine riboside, which can be used for the study of pharmacokinetics of N6-(4-hydroxybenzyl) adenine riboside.

Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
TANG Chunlan
WANG Li
CHENG Mengchun
LIU Xinxin
XIAO Hongbin
Key wordsultra performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS)   N6-(4-hydroxybenzyl) adenine riboside   pharmacokinetics   rat plasma     
Received: 2015-03-12;
Cite this article:   
TANG Chunlan,WANG Li,CHENG Mengchun et al. Determination of N6-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(7): 699-703.
 
No references of article
[1] WANG Pingya, ZHOU Yong, DAI Yifei, HUANG Li, ZHAO Qiaoling. Rapid screening of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(8): 822-829.
[2] GAO Meng, WANG Yuesheng, WEI Huizhen, OUYANG Hui, HE Mingzhen, ZENG Lianqing, SHEN Fengyun, GUO Qiang, RAO Yi. Qualitative and quantitative analysis of amygdalin and its metabolite prunasin in plasma by ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(6): 591-599.
[3] XIONG Shan, LI Jinglai, ZHU Xiuqing, WANG Xiaoying, LÜ Guiyuan, ZHANG Zhenqing. Determination of morroniside concentration in beagle plasma and its pharmacokinetics by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(3): 290-293.
[4] XU Cheng-Bao-1, 2 , SUO Ran-1*, ZHANG Feng-2*, CHU Xiao-Gang-2, DING Fei-2, 3 , LING Yun-2, YANG Min-Li-2, SUN Li-2. Simultaneous determination of 10 unapproved sedative drugs in feeds by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2012, 30(05): 457-462.
[5] ZHOU Lijun1,2, LI Jinglai2, WANG Xiaoying2, QIAO Jianzhong2, ZHANG Zhenqing2*. Determination of ilaprazole in beagle plasma and its pharmacokinetics by high performance liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2012, 30(05): 452-456.
[6] WU Xiaoyu1, WANG Rong1,2*, XIE Hua1, WANG Jianfeng1, JIA Zhengping1,2*, ZHANG Qiang1, WANG Xianhua1. Rapid determination of propranolol enantiomers in rat plasma by column-switching-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2011, 29(12): 1205-1209.
[7] XI Heng1, HAN Guozhu1, L Li1*, ZHANG Di2. Simultaneous determination of exogenous phosphocreatine and its metabolite creatine in rabbit plasma using ion-pair reversed-phase high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2011, 29(10): 1000-1004.
[8] XIN Yang1,2, PI Zifeng1, SONG Fengrui1, LIU Zhiqiang1, LIU Shuying1*. Comparison of intracorporal absorption of hypaconitine in Heishunpian decoction and its compound recipe decoction by ultra performance liquid chromatography-quadrupole time- of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2011, 29(05): 389-393.
[9] CHANG Xiaojuan, PENG Jingdong, LIU Shaopu, LIU Limin, DAI Yongkuang. Determination of netilmicin in rat plasma by reversed-phase high performance liquid chromatography with fluorescence detection and pre-column derivatization[J]. Chinese Journal of Chromatography, 2009, 27(6): 794-798.
[10] WU Jianlin, YUE Hao, CAI Zongwei. Investigation on metabolism and pharmacokinetics of triclosan in rat plasma by using UPLC-triple quadrupole MS[J]. Chinese Journal of Chromatography, 2009, 27(5): 724-730.
[11] HAN Ying, XIONG Zhili, YANG Chunjuan, LIU Man, LI Famei. Determination of Protocatechuic Acid in Rat Plasma by High Performance Liquid Chromatography[J]. Chinese Journal of Chromatography, 2007, 25(2): 207-210.
[12] ZHAO Jingshan, WEN Jinkun, HAN Mei. Detection of Acetylbritannilactone in Inulicin Ingredients and Dynamic Change in Vivo by Micellar Electrokinetic Capillary Chromatography[J]. Chinese Journal of Chromatography, 2006, 24(5): 508-512.
[13] ZHANG Wei, YANG Binghu, LIANG Qiande, LU Dandan, LIN Ruxian, WANG Shengqi . Quantitative Analysis of Cantide in Plasma by Capillary Gel Electrophoresis[J]. Chinese Journal of Chromatography, 2005, 23(4): 374-377.
[14] WANG Rui, SUN Tianhui, JING Dan, CHEN Xiaohui, YU Zhiguo, BI Kaishun . High Performance Liquid Chromatographic Determination of Cinnamic Acid in Rabbit Plasma and[J]. Chinese Journal of Chromatography, 2005, 23(3): 273-275.
[15] . [J]. Chinese Journal of Chromatography, 2003, 21(3): 251-254.
?

Copyright © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS   辽ICP备10003855号
457 Zhongshan Road,116023, Dalian,P.R.China
Tel: +86-411-84379021, Fax: +86-411-84379600    E-mail: sepu@dicp.ac.cn

Supported by:Beijing Magtech

美女图片

美女

美女图片