[an error occurred while processing this directive]
Chinese Journal of Chromatography
   Home   |   About Journal   |   Editorial Board   |   Impact Factor   |   Indexed In   |   Aims and Scope   |   Download   |   Subscriptions   |   FAQ   |   Contact Us   |   Chinese
Chinese Journal of Chromatography  2015, Vol. 33 Issue (9): 974-980    DOI: 10.3724/SP.J.1123.2015.04028
Current Issue | Next Issue | Archive | Adv Search  |   
Determination of flavonol glycosides in tea samples by ultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry
WANG Zhicong1,2, SHA Yuebing1, YU Xiaobo1, LIANG Yuerong2
1. Zhejiang Province Institute of Metrology, Hangzhou 310018, China;
2. Tea Research Institute, Zhejiang University, Hangzhou 310058, China
 Download: [PDF 1362KB]   [Full Text(HTML)] ()   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 

An ultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry (UPLC-PDA-MS/MS) method was developed for the determination of flavonol glycosides in tea samples. The chromatographic separation was performed on an UPLC HSS T3 column by gradient elution with the mobile phases of acetonitrile and water both containing 0.1%(v/v) formic acid. A total of 15 flavonol glycosides which include 3 myricetin glycosides, 6 quercetin glycosides and 6 kaempferol glycosides were positively identified in green and black tea samples by comparing the retention times and mass spectra of the samples with standards and publications. The quantities of flavonol glycosides were relatively calculated with the standard quercetin-3-rhamnosylglucoside (Q-GRh) which was calibrated with external quantification method using multi-reaction monitoring (MRM) mode. The results showed that there were different flavonol glycoside distributions in green tea and black tea. The total amount of flavonol glycosides in green tea was 1.7 times of that in black tea. The major flavonol glycosides in green tea were myricetin-3-galactoside (M-Ga), myricetin-3-glucoside (M-G), quercetin-3-glucosyl-rhamnosyl-galactoside (Q-GaRhG), quercetin-3-glucosyl-rhamnosyl-glucoside (Q-GRhG), kaempferol-3-glucosyl-rhamnosyl-galactoside (K-GaRhG) and kaempferol-3-glucosyl-rhamnosyl-glucoside (K-GRhG), but for black tea, the major flavonol glycosides were quercetin-3-rhamnosylglucoside (Q-GRh), quercetin-3-glucoside (Q-G), kaempferol-3-rhamnosylglucoside (K-GRh) and kaempferol-3-galactoside (K-Ga). The present method is accurate, convenient for the rapid identification of flavonol glycosides and analysis of constituent distribution for green and black teas.

Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WANG Zhicong
SHA Yuebing
YU Xiaobo
LIANG Yuerong
Key wordsultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry (UPLC-PDA-MS/MS)   flavonol glycosides   teas     
Received: 2015-04-22;
Cite this article:   
WANG Zhicong,SHA Yuebing,YU Xiaobo et al. Determination of flavonol glycosides in tea samples by ultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(9): 974-980.
 
No references of article
[1] YU Lu, SONG Wei, LÜ Yaning, ZHAO Muyu, ZHOU Fangfang, HU Yanyun, ZHENG Ping. Rapid determination of 204 pesticide residues in tea by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(6): 597-612.
[2] HU Yeqin, XI Cunxian, CAO Shurui, WANG Guomin, LI Xianliang, ZHANG Lei, ZHANG Yunhuai. Simultaneous determination of 16 organophosphorous pesticides in vegetables, fruits and tea by gas chromatography coupled with clean-up by mesoporous alumina as solid-phase extraction adsorbent[J]. Chinese Journal of Chromatography, 2014, 32(7): 784-788.
[3] LIN Yonghui*, LIU Zhengcai, YANG Fang, QIU Yuanjin, LIU Suzhen, SU Zhijiao, ZHANG Qiong, XUE Zhimin, FANG Yu. Determination of glufosinate residue in tea by liquid chromatography-tandem mass spectrometry coupled with precolumn derivatization[J]. Chinese Journal of Chromatography, 2012, 30(12): 1260-1264.
[4] HU Beizhen1*, CAI Haijiang2, SONG Weihua1. Determination of eight pesticide residues in tea by liquid chromatography-tandem mass spectrometry and its uncertainty evaluation[J]. Chinese Journal of Chromatography, 2012, 30(09): 889-895.
[5] ZHANG Jialing, ZHANG Wei, ZHOU Zhigui, BAI Yu, LIU Huwei*. n situ and rapid identification of tea by direct analysis in real time mass spectrometry[J]. Chinese Journal of Chromatography, 2011, 29(07): 681-686.
[6] ZHOU Yu, XU Dunming*, CHEN Dajie, ZHANG Zhigang, ZHENG Xianghua, FANG Enhua. Determination of fipronil and its metabolites in tea by solid-phase microextraction coupled with gas chromatography and gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2011, 29(07): 656-661.
[7] LI Junming, ZHONG Dubo, WANG Yaqin, FENG Lei, ZHU Hongkun. Determination of 153 pesticide residues in tea using on-line gel permeation chromatography-gas chromatography/mass spectrometry[J]. Chinese Journal of Chromatography, 2010, 28(09): 840-848.
[8] CHEN Linglong, CHEN Jiuxing, MA Ming, CHEN Lihua, YANG Hui, ZHANG Guiqun. Determination of lambda-cyhalothrin residue in tea and soil using gas chromatography[J]. Chinese Journal of Chromatography, 2010, 28(08): 817-820.
[9] WANG Qingping, ZHANG Lan, CHEN Guonan, LIN Jin-Ming. Determination of four amino acids in tea by capillary zone electrophoresis and direct ultraviolet detection without derivatization[J]. Chinese Journal of Chromatography, 2009, 27(6): 840-844.
[10] YAN Hongfei, HUANG Zhiqiang, ZHANG Ying, LI Yongjun, WANG Meiling. Determination of 29 acidic herbicide residues in tea by gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2009, 27(3): 288-293.
[11] YANG Fang, YANG Shoushen, LIN Yonghui, ZHENG Danping, LU Shengyu, HUANG Zhiqiang, ZHANG Ying. Determination of avermectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2009, 27(2): 153-157.
[12] LOU Zhengyun, CHEN Zongmao, LUO Fengjian, TANG Fubin, LIU Guangming. Determination of 92 pesticide residues in tea by gas chromatography with solid-phase extraction[J]. Chinese Journal of Chromatography, 2008, 26(5): 568-576.
[13] ZHAN Jiafen, LU Sheming , MENG Zhaoyu, XIANG Nengjun,CAO Qiu’e, MIAO Mingming.

Analysis of volatile components in Qingshanlvshui Tea using solid-phase microextraction/accelerated solvent
extraction-gas chromatography-mass spectrometry

[J]. Chinese Journal of Chromatography, 2008, 26(3): 301-305.
[14] HU Beizhen, SONG Weihua, XIE Liping, SHAO Tiefeng.

Determination of 33 pesticides in tea by accelerated solvent extraction-gel permeation and solid-phase extraction
purification-gas chromatography-mass spectrometry

[J]. Chinese Journal of Chromatography, 2008, 26(1): 22-28.
[15] SHEN Weijian, XU Jinzhong, YANG Wenquan, SHEN Chongyu, ZHAO Zengyun, DING Tao, WU Bin.

Determination of Acetanilide Herbicide Residues in Tea by Gas

Chromatography-Mass Spectrometry with Two Different Ionization Techniques

[J]. Chinese Journal of Chromatography, 2007, 25(5): 753-757.
?

Copyright © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS   辽ICP备10003855号
457 Zhongshan Road,116023, Dalian,P.R.China
Tel: +86-411-84379021, Fax: +86-411-84379600    E-mail: sepu@dicp.ac.cn

Supported by:Beijing Magtech

美女图片

美女

美女图片