Chinese Journal of Chromatography ›› 2017, Vol. 35 ›› Issue (3): 229-236.DOI: 10.3724/SP.J.1123.2016.10040

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Progress in strategies for enrichment of proteome terminal peptides

CHEN Lingfan1,2, SHAN Yichu1, ZHANG Lihua1, ZHANG Yukui1   

  1. 1. Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-10-18 Online:2017-03-08 Published:2013-07-16
  • Supported by:

    National Natural Science Foundation of China (Nos. 21475127, 21235005); National Important Project on Science Instrument (No. 2012YQ120044-8).

Abstract:

Termini of proteins are not only the origin and destination of protein synthesis, but also participating in and mediating a host of physiological functions of proteins. The determination of proteins termini provides valuable information for protein structure and function annotation, and helps the profiling of proteases substrates and cleavage sites. Herein, the progress in the strategies for enrichment of protein terminus is reviewed. In the meantime, the applications of terminome analysis for the identification of protease substrate and cleavage site are briefly introduced. Finally, the future of terminome research is discussed.

Key words: protease substrate and cleavage site, review, termini enrichment, terminome

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