Fast detection of kanamycin in milk by protamine-aptamer-gold nanotechnology

doi:10.3724/SP.J.1123.2016.10007

Abstract

Abstract:

A bio-nanodetection system was developed for kanamycin based on electrostatic interaction between polycationic protamine and negatively charged aptamer or gold nanoparticles (AuNPs). The concentrations of protamine, aptamer and cations in the buffer solution were optimized. The results showed that there was a good linear correlation (R²=0.9928) between gold nanoparticles and kanamycin in the range of 5-5000 nmol/L with 20 mmol/L Na⁺, 1 mmol/L Mg²⁺, 2 mg/L protamine and 100 nmol/L aptamer. The limit of detection was 0.53 nmol/L. Under this experimental condition, the content of kanamycin in milk was detected, the recoveries were 96%-98%, and the relative standard deviations (RSDs) were 1.5%-3.2%. This method has the advantages of high selectivity, good sensitivity and wide linear range, and is useful in the detection of kanamycin in foods.

Key words: aptamer, bio-nano, gold nanoparticles, kanamycin, milk, protamine

CLC Number:

O658

JIA Xiangyang, YOU Huiyan, FU Xiuli. Fast detection of kanamycin in milk by protamine-aptamer-gold nanotechnology[J]. Chinese Journal of Chromatography, 2017, 35(3): 269-273.

References

[1] Megoulas N C, Koupparis M A. Anal Chim Acta, 2005, 547: 64
[2] Chen P Z, Lin X C, Ma C H, et al. Journal of Instrumental Analysis, 2008, 27(12): 1367 陈培珍, 林旭聪, 马春花, 等. 分析测试学报, 2008, 27(12): 1367
[3] Gutierrez-Huante M, Martinez H, Bustamante V H, et al. Lett Appl Microbiol, 2015, 60: 440
[4] Kaufmann A, Butcher P, Maden K. Anal Chim Acta, 2012, 711: 46
[5] Bogialli S, Cuini R, Di Corcia A, et al. J Chromatogr A, 2005, 1067: 93
[6] Wei Q, Zhao Y F, Du B, et al. Food Chem, 2012, 134: 1601
[7] Wang Y, Ji S, Zhang F, et al. J Chromator A, 2015, 1403: 32
[8] Yan J L, Sun R D. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2002, 38(9): 446 严金龙, 孙汝东. 理化检测-化学分册, 2002, 38(9): 446
[9] Gong Q, Ding L, Zhu S H, et al. Chinese Journal of Chromatography, 2012, 30(11): 1143 龚强, 丁利, 朱绍华, 等. 色谱, 2012, 30(11): 1143
[10] Liu Z Z, Li W D, Wang J Q. Capital Food and Medicine, 2015(4): 46 刘照振, 李文东, 王俊秋. 首都食品与医药, 2015(4): 46
[11] Sun X, Li F L, Shen G H, et al. Anal, 2014, 139: 299
[12] Zhu Y, Chandra P, Song K M, et al. Biosens Bielectron, 2012, 36: 29
[13] Ge S L, Tang W R, Han R B, et al. J Chromatogr A, 2013, 1295: 128
[14] Zhang X Y, Cai X X. Chinese Journal of Chromatography, 2014, 32(7): 693 张秀尧, 蔡欣欣. 色谱, 2014, 32(7): 693
[15] Wang H, Zhao L, Yang H M, et al. Chinese Journal of Chromatography, 2015, 33(9): 995 王浩, 赵丽, 杨红梅, 等. 色谱, 2015, 33(9): 995
[16] Li W, Ai L F, Guo C H, et al. Chinese Journal of Chromatography, 2013, 31(10): 946 李玮, 艾连峰, 郭春海, 等. 色谱, 2013, 31(10): 946
[17] Wang Y, Zhao X Y, Shi D D, et al. Chinese Journal of Chromatography, 2016, 34(4): 361 王勇, 赵新颖, 石冬冬, 等. 色谱, 2016, 34(4): 361
[18] Robati R Y, Arab A, Ramezani M, et al. Biosens Bioelectron, 2016, 82: 162
[19] Luo J B. [PhD Dissertation]. Wuxi: Jiangnan University, 2015 罗继宝. [博士学位论文]. 无锡: 江南大学, 2015
[20] Chen X L, Zhang H, Liu B, et al. Journal of Instrumental Analysis, 2015, 33(6): 709 陈效兰, 张慧, 刘冰, 等. 分析测试学报, 2015, 33(6): 709
[21] Xu F, Zhou J, Wu Y, et al. Scientia Agricultura Sinica, 2015, 48(21): 4358 徐飞, 周洁, 吴越, 等. 中国农业科学, 2015, 48(21): 4358
[22] Fu X L, Chen L X, Li J H. Analyst, 2012, 137: 3653
[23] Frens G. Nat J Phy Sci, 1973, 241: 20
[24] Zhou N D, Zhang J, Tian Y P. Anal Methods, 2014, 6: 1569
[25] Wang C K, Chen D, Lu F, et al. Journal of Instrumental Analysis, 2015, 34(12): 1434 王承克, 陈丹, 陆峰, 等. 分析测试学报, 2015, 34(12): 1434
[26] Wang S S. [PhD Dissertation]. Yantai: University of Chinese Academy of Sciences, 2016 王莎莎. [博士学位论文]. 烟台: 中国科学院大学, 2016
[27] Mao Y Q, Hu M N, Li N, et al. Journal of Analytical Science, 2016, 32(2): 253 毛永强, 胡美娜, 李娜, 等. 分析科学学报, 2016, 32(2): 253
[28] Yu C Z, He G N, Xie H Y, et al. J Chromatogr B, 2009, 877: 333