Investigation on interactions between bovine thrombin, aptamer and active components in Danshen by capillary zone electrophoresis

doi:10.3724/SP.J.1123.2016.10018

Abstract

Abstract:

Investigation on interactions between bovine thrombin (B-Thr), aptamer29 (Apt29) and three active components (salvianic acid A sodium (SAS), protocatechuic acid (PA), ferulic acid (FA)) in Danshen was carried out by capillary zone electrophoresis (CZE). CZE conditions were as follows: capillary length of 40 cm, voltage of 15 kV, injection volume of 3.45 kPa, injection time of 5 s. By changing concentration of B-Thr or Apt29 with fixed levels of the three active components, binding constants (K_b) were calculated by Scatchard and non-specific binding equations to show interactions between B-Thr, Apt29 and the three active components. The results showed that PA had the strongest affinity to B-Thr with K_b 3.39×10⁴L/mol. K_b between FA and B-Thr was 1.05×10⁴ L/mol. SAS had no binding with B-Thr. FA showed stronger affinity with Apt29 than PA with K_b of 1.48×10⁴ L/mol and 1.32×10⁴ L/mol, respectively. The present study reported that interactions between the active constituents in Danshen and B-Thr/Apt29. New methods might be supplied for calculation of interactions between Apt (protein) and herbal drug molecules. And it could give insight for target discovery and delivery of herbal drug molecules.

Key words: aptamer (Apt29), bovine thrombin (B-Thr), capillary zone electrophoresis (CZE), Danshen

CLC Number:

O658

LI Yujuan, WU Guangxia, OU Wanlu, SUN Xinxin, QU Feng. Investigation on interactions between bovine thrombin, aptamer and active components in Danshen by capillary zone electrophoresis[J]. Chinese Journal of Chromatography, 2017, 35(3): 339-343.

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