Abstract:

An effective analytical method based on supercritical fluid chromatography (SFC) was developed for the simultaneous determination of eight allergenous disperse dyes (disperse yellow 1, disperse yellow 49, disperse yellow 9, disperse orange 37/76, disperse red 1, disperse orange 1, disperse orange 3 and disperse brown 1). Blended carpet samples with various colours were ultrasonically extracted for 30 min with methanol at 70℃. Then the extracts were filtrated by 0.45 μm filters. Under the optimum conditions of supercritical CO₂-methanol modifier (90:10, v/v) as mobile phase, 440 nm as UV detection wavelength, the eight allergenous disperse dyes were separated completely within 12 min. The eight allergenous disperse dyes exhibited satisfactory linearity with correlation coefficients (r²) of 0.9992-0.9998. The limits of detection (LOD, S/N=3) were 0.05-0.10 mg/L. The developed method was validated for the determination of the eight allergenous disperse dyes in blended carpets with good spiked recoveries ranged from 95.6% to 104.2%. The relative standard deviations (RSDs) of the peak areas of the eight allergenous disperse dyes were all less than 1.2% (n=5). The method is convenient, rapid, sensitive, accurate, selective, and adaptable to the application for detecting the eight allergenous disperse dyes in blended carpets.

Key words: allergenous disperse dyes, blended carpet, supercritical fluid chromatography (SFC), ultraviolet detection