Simultaneous determination of five mycotoxins in various grains and their products by liquid chromatography-tandem mass spectrometry with isotope internal standard

doi:10.3724/SP.J.1123.2016.12028

Abstract

Abstract:

A rapid method was established for the determination of zearalenone (ZON), nivalenol (NIV), deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-ACDON) and 15-acetyl-deoxynivalenol (15-ACDON) in various grains and their products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted by acetonitrile-water (84:16, v/v), and cleaned-up by multifunctional column without any further step. The separation was carried out on an Acquity UPLC^® BEH C18 column (100 mm×2.1 mm, 1.7 μm) with gradient elution using acetonitrile-water as mobile phases. The analytes were quantified by isotope internal standard method. The correlation coefficients (r²) were all above 0.99. The limits of detection (LODs, S/N=3) ranged from 5 μg/kg to 20 μg/kg. The recoveries of the five mycotoxins spiked in blank grain and its product samples varied from 84.2% to 114.5%, with the relative standard deviations (RSDs) of 0.4%-9.9% (n=6). The method is accurate, efficient, sensitive and practical.

Key words: grains and their products, isotope internal standard, liquid chromatography-tandem mass spectrometry (LC-MS/MS), mycotoxins

CLC Number:

O658

GAO Bei, JIANG Deming, YANG Yongtan. Simultaneous determination of five mycotoxins in various grains and their products by liquid chromatography-tandem mass spectrometry with isotope internal standard[J]. Chinese Journal of Chromatography, 2017, 35(6): 601-607.

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