Fabrication of acylsemicarbazide-based porous organic polymer for selective enrichment of glycopeptides

doi:10.3724/SP.J.1123.2017.05014

Abstract

Abstract:

An acylsemicarbazide-based porous organic polymer (POP) was facilely prepared by the polymerization of benzene-1,3,5-tricarbohydrazide (BTZ) and 1,4-phenylene diisocyanate (PDI). The physical properties of as-synthesized material were characterized by Fourier-transform infrared spectroscopy (FT-IR), solid-state cross polarisation magic angle spinning carbon-13 nuclear magnetic resonance (CP-MAS ¹³C NMR), nitrogen adsorption/desorption measurement, water contact angle and so on. The specific surface area was 166 m²/g, and the water contact angle was 46.4°, exhibiting hydrophilic property of the porous material. Thus, POP-1 was tried to be used for glycopeptides enrichment from tryptic digest of standard protein and complex biosamples in hydrophilic mode. The 19 typical N-linked glycopeptides were identified from 500 fmol tryptic digest of immunoglobulin G (IgG) from human serum by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis, meanwhile, 1919 unique glycopeptides with 1350 N-glycosylation sites from 605 different N-linked glycoproteins were identified from 100 μ g mouse liver tryptic digest by capillary liquid chromatography (cLC)-MS/MS analysis. These results demonstrated the great potential of POP-1 for glycoproteome analysis.

Key words: 1,4-phenylene diisocyanate (PDI), acylsemicarbazide, benzene-1,3,5-tricarbohydrazide (BTZ), glycopeptide enrichment, hydrophilic, porous organic polymer

CLC Number:

O658

WANG Hongwei, LIU Zhongshan, PENG Xiaojun, OU Junjie, YE Mingliang. Fabrication of acylsemicarbazide-based porous organic polymer for selective enrichment of glycopeptides[J]. Chinese Journal of Chromatography, 2017, 35(7): 688-695.

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