High-sensitive detection of multiple allergenic proteins in infant food with high-resolution mass spectrometry

doi:10.3724/SP.J.1123.2017.06013

Chinese Journal of Chromatography ›› 2017, Vol. 35 ›› Issue (10): 1037-1041.DOI: 10.3724/SP.J.1123.2017.06013

High-sensitive detection of multiple allergenic proteins in infant food with high-resolution mass spectrometry

WU Ci¹, CHEN Xi¹, LIU Jianhui^2,3, ZHANG Xiaolin¹, XUE Weifeng¹, LIANG Zhen², LIU Mengyao¹, CUI Yan¹, HUANG Daliang¹, ZHANG Lihua²

1. Inspection and Quarantine Technical Center of Dalian Entry-Exit Inspection and Quarantine Bureau, Dalian 116100, China;
2. Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
3. University of the Chinese Academy of Sciences, Beijing 100049, China

Received:2017-06-07 Online:2017-10-08 Published:2017-10-18
Supported by:
Open Fund Supported by Key Laboratory of Separation Sciences for Analytical Chemistry, Chinese Academy of Sciences (No. KL1606); Science and Technology Project of State General Administration of the People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK343).

Abstract

Abstract:

A novel method of the simultaneous detection of multiple kinds of allergenic proteins in infant food with parallel reaction monitoring (PRM) mode using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established. In this method, unique peptides with good stability and high sensibility were used to quantify the corresponding allergenic proteins. Furthermore, multiple kinds of allergenic proteins are inspected simultaneously with high sensitivity. In addition, such method was successfully used for the detection of multiple allergenic proteins in infant food. As for the sample preparation for infant food, compared with the traditional acetone precipitation strategy, the protein extraction efficiency and capacity of resisting disturbance are both higher with in-situ filter-aided sample pretreatment (i-FASP) method. All allergenic proteins gave a good linear response with the correlation coefficients (R²) ≥ 0.99, and the largest concentration range of the allergenic proteins could be four orders of magnitude, and the lowest detection limit was 0.028 mg/L, which was better than that reported in references. Finally, the method was conveniently used to detect the allergens from four imported infant food real samples. All the results demonstrate that this novel strategy is of great significance for providing a rapid and reliable analytical technique for allergen proteomics.

Key words: allergenic protein, infant food, liquid chromatography-tandem mass spectrometry (LC-MS/MS), parallel reaction monitoring (PRM)

CLC Number:

O658

WU Ci, CHEN Xi, LIU Jianhui, ZHANG Xiaolin, XUE Weifeng, LIANG Zhen, LIU Mengyao, CUI Yan, HUANG Daliang, ZHANG Lihua. High-sensitive detection of multiple allergenic proteins in infant food with high-resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(10): 1037-1041.

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High-sensitive detection of multiple allergenic proteins in infant food with high-resolution mass spectrometry

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