Determination of 14 sweeteners in liquid foods by hydrophilic interaction liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2017.10023

Abstract

Abstract:

A method for the simultaneous determination of six artificial sweeteners and eight steviol glycosides in liquid food samples by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) was developed.The sweeteners in the samples were separated by a Waters Xbridge Amide column (150 mm×5.0 mm, 3.5 μm) at 35℃, and eluted with acetonitrile-10 mmol/L ammonium formate (65:35, v/v) at a flow rate of 0.4 mL/min.Then, the analytes were detected using an electrospray ion source in negative mode (ESI^-) under multiple reaction monitoring (MRM) mode.Good linear relationships were obtained, and the correlation coefficients (r²) of the 14 sweeteners were greater than 0.995.The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) ranged from 0.03 to 0.7 mg/kg and 0.1 to 2.2 mg/kg, respectively.The average recoveries varied from 80.8% to 108.7% at spiked levels of 2, 5 and 20 mg/kg, and the relative standard deviations (RSDs) ranged from 1.5% to 7.7%(n=6).The proposed method is simple, accurate and sensitive for the simultaneous determination of the 14 sweeteners in liquid food samples.

Key words: artificial sweeteners, hydrophilic interaction liquid chromatography (HILIC), liquid food, steviol glycosides, triple quadrupole mass spectrometry

CLC Number:

O658

FAN Guangyu, FENG Feng, ZHANG Feng, GAO Fei, LI Xiaoming, LIANG Zhengang. Determination of 14 sweeteners in liquid foods by hydrophilic interaction liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(4): 351-355.

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