Chinese Journal of Chromatography ›› 2018, Vol. 36 ›› Issue (4): 381-387.DOI: 10.3724/SP.J.1123.2017.11014

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Study on effect of ionic strength on photolysis of vitamin B2 based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection

XIAO Han1, Kevin YAN2, ZHENG Yiting1, WANG Yan1, YAN Chao1   

  1. 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China;
    2. University of California, Riverside 92521, USA
  • Received:2017-11-09 Online:2018-04-08 Published:2014-02-26
  • Supported by:

    Shanghai Science and Technology Commission Scientific Research Project (Nos. 16142200300, 17142201000); Shanghai Introduction of Technology Absorption and Innovation (No. XC-ZXSJ-02-2016-11); Shanghai Standardization Promotion Project (No. 17DZ2201500).

Abstract:

A new method for the separation and analysis of vitamin B2 and its fluorescent photolysis products was established based on pressurized capillary electrochromatography (pCEC) coupled with laser induced fluorescence (LIF) detection.The relationship between the photolysis reaction rate of vitamin B2 and the ionic strength of aqueous solution or phosphate buffered solution was studied.The analysis was carried out by using a C18 capillary column.The mobile phase was acetonitrile-water with 0.1%(v/v) trifluoroacetic acid with gradient elution.The excitation wavelength was 488 nm and the emission wavelength was 520 nm.Vitamin B2 and many fluorescent photolysis products were well separated.Based on the above, the influence of photo degradation rate of vitamin B2 with ionic strength was studied.The apparent rate constant of photolysis was calculated by kinetic calculation.The results showed that the ionic strength had a significant effect on the photolysis of vitamin B2 solution.The photolysis rate of vitamin B2 was positively related to the ionic strength.This study provides a highly efficient method for the stability research of vitamin B2, which may serve as a good reference for the preservation and clinical use of it.

Key words: laser induced fluorescence detection (LIF), photolysis reaction, pressurized capillary electrochromatography (pCEC), reaction rate constant, vitamin B2

CLC Number: