Simultaneous chiral separation of three triazole pesticide enantiomers with submicron C18 bonded silica stationary phase by pressurized capillary electrochromatography

doi:10.3724/SP.J.1123.2017.11015

Abstract

Abstract:

A monodispersed C18 silica stationary phase with particle size of 420 nm was prepared by modified St ber method.The column packed with submicron C18 bonded silica was successfully used for simultaneous enantioseparation of three chiral triazole pesticides by pressurized capillary electrochromatography (pCEC).Some effects on separation were investigated, and the optimized conditions were as follows:mobile phase of acetonitrile and 20 mmol/L phosphate buffer (pH=6.8) containing 20 mmol/L (2-hydroxypropyl)-γ-cyclodextrin (HP-γ-CD)(45:55, v/v), flow rate of 0.04 mL/min and applied voltage of-9.4 kV, the detection wavelength at 220 nm.The resolutions of adjacent peaks of uniconazole, diniconazole and propiconazole enantiomers were 4.20, 12.9, 4.41, 4.09 and 1.70 in 12 min, respectively, with the column efficiency up to 310000 plates/m.This study provides an alternative way for the simultaneous separation of chiral triazole pesticides with high efficiency.

Key words: (2-hydroxypropyl)-γ-cyclodextrin (HP-γ-CD), chiral mobile phase additives, chiral triazole pesticide, pressurized capillary electrochromatography (pCEC), submicron silica particles

CLC Number:

O658

CHEN Qiaomei, LIU Qing, SHEN Lin, XUE Yun, WANG Yan, YAN Chao. Simultaneous chiral separation of three triazole pesticide enantiomers with submicron C18 bonded silica stationary phase by pressurized capillary electrochromatography[J]. Chinese Journal of Chromatography, 2018, 36(4): 388-394.

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