Chinese Journal of Chromatography ›› 2018, Vol. 36 ›› Issue (4): 408-412.DOI: 10.3724/SP.J.1123.2017.11002

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Determination of the ingredients of horse, ox, sheep, pig, camel and deer skin in colla corii asini by ultra performance liquid chromatography-tandem mass spectrometry

HANG Baojian1, TIAN Chenying2, CHEN Xiao1, XING Sheng1, SHI Feng1, LENG Jiawei1, GONG Liping1   

  1. 1. Shandong Institute for Food and Drug Control, Jinan 250101, China;
    2. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2017-11-03 Online:2018-04-08 Published:2014-02-26
  • Supported by:

    Major New Drug Creation Program of China (No. 2014ZX0930437).

Abstract:

A method was established for detecting the ingredients of horse, ox, sheep, pig, camel and deer skin in colla corii asini using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Theoretical marker peptides were selected by comparing the collagen sequences in the skins of donkeys, horses, oxen, sheep, pigs, camels, and deer.Marker peptides were identified by protease cutting techniques and high resolution mass spectrometry and then analyzed by UPLC-MS/MS.The separation was performed on a UPLC system with a BEH C18 column via the gradient elution of acetonitrile containing 0.1%(v/v) acetic acid and water containing 0.1%(v/v) acetic acid.The marker peptides were identified in the modes of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM).The marker peptides for the horse, ox, sheep, pig and camel skin were detected in 15 samples.The method is simple, highly reproducible, and suitable for the identification of mixed skin ingredients in colla corii asini.It has been successfully used to detect the authenticity of colla corii asini.

Key words: colla corii asini, collagen, marker peptide, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number: