Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2018, Vol. 36 ›› Issue (5): 464-473.DOI: 10.3724/SP.J.1123.2017.12025

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Separation of lignans from Schisandra chinensis by two-dimensional reversed-phase liquid chromatography

ZHU Jingbo1,2, LI Wenyu1, XIAO Wei2, DING Yan1, HUANG Wenzhe2, TU Pengfei2,3, WANG Yonghua2,4   

  1. 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;
    2. Jiangsu Kanion Pharmaceutical Corporation Limited, Lianyungang 222001, China;
    3. Peking University, Beijing 100871, China;
    4. Northwest Agriculture and Forestry University, Yangling 712100, China
  • Received:2017-12-27 Online:2018-05-08 Published:2014-03-21
  • Supported by:

    Project of Joint Fund of the National Natural Science Foundation of China (No. U1603285); Basic Scientific Research Project of Education Department of Liaoning Province of China (No. 2016J008); "Double" Project of Jiangsu Province of China (Jiangsu Talent No.[2015]26); "High Level Innovation and Entrepreneurial Talent Gathering Project" in Lianyungang City of China (Lianyungang Talent No.[2016]1).

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Abstract:

A two-dimensional reversed-phase liquid chromatography (2D-RPLC) method was established to separate lignan components and pure compounds from Schisandra chinensis. This method was based on the modules of separation-enrichment and self-developed with independent intellectual property rights. First, a C18 column (250 mm×4.6 mm, 5 μm) was used as the analytical column. A water-methanol mixture was selected as the mobile phase. Separation resolution and retention time were selected as indexes. Gradient chromatography of the lignan extracts was repeated four times and the information was input into XTool, a chromatographic separation software. The conditions for the first-and second-dimensional chromatographic separation were optimized by XTool. Then, linear amplification was used for preparative chromatography. C18 column (250 mm×30 mm, 10 μm) was selected as the first-and second-dimensional separation column. The C18 columns (80 mm×30 mm, 10 μm) were selected as enriching columns, and water was chosen as the diluent solution. Lignans from Schisandra chinensis were separated and purified by 2D-RPLC. Finally, nine lignan components were obtained by the first-dimensional separation. Totally, 20 high-purity compounds, containing six monomers, were obtained by the second-dimensional separation. Experimental results showed that this method has good reproducibility. This method thus allows for the systematic separation of lignans from Schisandra chinensis.

Key words: Schisandra chinensis, chromatographic expert system software simulation, lignans, two-dimensional reversed-phase liquid chromatography (2D-RPLC)

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