Rat plasma metabolomics in blood stasis model based on ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry

doi:10.3724/SP.J.1123.2018.09008

Chinese Journal of Chromatography ›› 2019, Vol. 37 ›› Issue (1): 71-79.DOI: 10.3724/SP.J.1123.2018.09008

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Rat plasma metabolomics in blood stasis model based on ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry

YANG Xiujuan^1,2, YANG Zhijun^1,2, LI Shuo^1,2, DENG Yi^1,2, YANG Yanze¹, MAN Qiong¹, LI Pengjie¹

1. Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;
2. Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, China

Received:2018-09-05 Online:2019-01-08 Published:2014-11-29
Supported by:
National Natural Science Foundation of China, Regional Science Foundation Project (No. 81360633); Gansu Provincial Natural Science Foundation of China (No. 1506RJZA044); Research Project Fund Project of Gansu Provincial Administration of Traditional Chinese Medicine (No. GZK-2016-25); Scientific Research Project of the Higher Education Institutions of Gansu Province (Nos. 2017A-056, 2016B-055); Research Fund Project of Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine of Gansu Province (No. zzy-2016-06); Research Fund Project of Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level (No. DHYX18-11).

Abstract

Abstract:

Acute blood stasis syndrome was induced in rats by adrenaline hydrochloride and ice water. Ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was conducted on plasma metabolites of normal and model rats. Principal component analysis (PCA), differentiation analysis of supervised partial least squares method (PLS-DA), and orthogonal to partial least squares discriminant analysis (OPLS-DA) on metabolomics data for multidimensional statistical analysis were employed, and the resulting biomarkers were screened. Compared to the normal group, there were 46 endogenous metabolites in blood stasis-rat plasma. Of these, 21 metabolites were significantly upregulated, such as acetylcholine, N6,N6,N6-trimethyl-L-lysine, cytosine, and acetylcarnitine, while 25 metabolites were reduced, including indoleacrylic acid, and lysoPC(14:0). These metabolites were mainly related to metabolic pathways, including lipid metabolism, galactose metabolism, linoleic acid metabolism, biosynthesis of unsaturated fatty acids, glycolysis, and arachidonic acid metabolism. In conclusion, these results indicated that metabolites could be used as important biomarkers for blood stasis syndrome, and could help in revealing the mechanism of blood stasis disease and provide a reference network to determine the disease development stage and appropriate follow-up treatment. Studying altered metabolites in blood stasis model rats can provide insights useful for the diagnosis of blood stasis in the clinic and for the development of drug therapies.

Key words: blood stasis syndrome, mechanism, metabolites, metabolomics, ultra performance liquid chromatography-quadrupole-time-of-flight mass spectro-metry (UPLC-Q-TOF/MS)

CLC Number:

O658

YANG Xiujuan, YANG Zhijun, LI Shuo, DENG Yi, YANG Yanze, MAN Qiong, LI Pengjie. Rat plasma metabolomics in blood stasis model based on ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(1): 71-79.

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Rat plasma metabolomics in blood stasis model based on ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry

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